无机聚酸盐通过激活P2X纯素受体来调节胸细胞的功能
Kamila S Nebesnaya1, Albert R Makhmudov2, Khondamir R Rustamov1
1Laboratory of Experimental Biophysics, Centre for Advanced Technologies, Tashkent, Uzbekistan; Department of Biophysics, Faculty of Biology, National University of Uzbekistan, Tashkent, Uzbekistan.
Biochimica et biophysica acta. General subjects
|November 25, 2023
概括
无机聚酸盐 (polyP) 作为胸细胞中的信号分子,激活P2X受体以增加细胞内和调节细胞体积. 这项研究揭示了polyP.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 无机聚酸盐 (polyP) 是一种已知在哺乳动物大脑中发出信号的古老聚合物.
- 它作为其他细胞类型中的素受体激素的作用在很大程度上仍未被探索.
- 以前的研究重点是通过天体细胞中的P2Y1受体传递polyP的信号.
研究的目的:
- 研究无机多酸盐 (polyP) 作为初级胸细胞中信号分子的作用.
- 确定参与这些免疫细胞中聚P介导信号传递的特定 purinoreceptor亚型.
- 为了阐明细胞中聚聚激活的下游生理效应.
主要方法:
- 原发性胸细胞用聚治疗,并测量细胞内的度.
- 评估了各种纯素受体抑制剂 (P2X,P2Y1) 对聚P诱导的信号的影响.
- 使用了线粒体去极化,细胞体积调节,分子对接和分子动力学模拟.
主要成果:
- 应用PolyP增加了胸细胞中的细胞内,依赖P2X但不是P2Y1受体.
- 聚P引起了轻微的线粒体脱极化,并以依赖的方式调节了细胞体积调节.
- 分子对接和动力学模拟表明,聚P与P2X受体结合,增加了水的透性.
结论:
- 这项研究首次证明,无机多酸盐 (polyP) 与胸细胞中的P2X受体相互作用.
- 聚作为P2X受体的激动剂,调节免疫细胞中的细胞内水平和生理过程.
- 这些发现扩展了聚的已知功能作为一个信号分子超出哺乳动物大脑.
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