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在干旱压力期间对ncRNAs和mRNAs相互作用的转录组分析

Cong Guan¹, Wei Li², Guoliang Wang¹

¹Institute of Leisure Agriculture, Shandong Academy of Agricultural Science, Jinan 250100, China; Key Laboratory of East China Urban Agriculture, Ministry of Agriculture, Jinan 250100, China; Shandong Engineering Research Center of Ecological and Horticultural Plant Breeding, Jinan 250100, China.

Plant science : an international journal of experimental plant biology

|November 25, 2023

概括

切换草干旱反应涉及多种不同的非编码RNA (ncRNA),如长非编码RNA (lncRNA) 和微RNA (miRNA). 这些ncRNAs调节粉代谢基因,通过复杂的调节网络增强干旱耐受性.

科学领域:

植物分子生物学植物分子生物学
基因组学和转录基因组学
生物能源作物研究生物能源作物研究

背景情况:

交换草 (Panicum virgatum L.) 是一种具有生态效益的关键生物能源作物.
非编码RNAs (ncRNAs) 对于植物应激反应至关重要,但它们在草干旱耐受性中的作用尚未得到充分研究.
了解ncRNA调节对于改善交换草的弹性至关重要.

研究的目的:

为了全面描述干旱压力下的交换草中mRNAs和ncRNAs (lncRNAs,circRNAs,miRNAs) 的表达特征.
阐明监管网络,包括竞争性内源性RNA (ceRNA) 网络,管理干旱应对.
为了研究特定基因,如粉合成酶4 (PvSS4) 在干旱耐受性中的功能.

主要方法:

在正常和干旱条件下,切换草叶的整个转录组序列.
对mRNAs,lncRNAs,circRNAs和miRNAs的差异表达分析.
KEGG路径分析,协同表达分析和ceRNA网络的构建.
通过在酵母中过度表达PvSS4的功能验证.

主要成果:

确定了数千种差异表达的mRNAs, lncRNAs, circRNAs和miRNAs.
上调的mRNA被丰富了粉和糖代谢途径.
构建了复杂的lncRNA/circRNA-miRNA-mRNA调节网络,突出的是PvSS4.
过度表达PvSS4增强了酵母的干旱耐受性,但没有盐耐受性.

结论:

不同的ncRNA在草干旱应激反应中起着重要作用.
涉及lncRNAs,circRNAs和miRNAs的ceRNA网络对于调节PvSS4.4等干旱适应基因至关重要.
PvSS4是提高草干旱耐受性的潜在目标.

关键词:

在CeRNA网络中,干旱造成的压力是干旱.小说_miRNA1222 的个人资料小说_miRNA14 在线阅读粉和糖的新陈代谢途径粉合成酶4 (PvSS4) 的作用切换草地切换草地

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