基因组挖掘揭示了昆虫病原菌细菌中的新生物合成基因集群
Wipanee Meesil1, Paramaporn Muangpat1, Sutthirat Sitthisak1,2
1Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.
Scientific reports
|November 25, 2023
概括
昆虫病原细菌Xenorhabdus和Photorhabdus的基因组挖掘揭示了新的生物合成基因集群 (BGCs). 这些集群,包括非核糖体合成酶 (NRPS) 和I型多基合成酶 (T1PKS),可能产生用于治疗和农业化学品的新生物活性化合物.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 生物技术是生物技术.
背景情况:
- 微生物天然产品对于开发新疗法和农业化学品至关重要.
- 昆虫病原性细菌,如Xenorhabdus和Photorhabdus,是生物活性化合物的丰富生产者.
研究的目的:
- 通过基因组挖掘探索Xenorhabdus和Photorhabdus细菌的生物合成潜力.
- 确定编码潜在生物活性二次代谢物的新生物合成基因集群 (BGCs).
主要方法:
- 使用下一代测序 (NGS) 来获取细菌基因组.
- 生物信息学工具被用于基因组挖掘和BGCs的识别.
- 进行了对比基因组分析,以评估已识别的BGCs的新性.
主要成果:
- 在Xenorhabdus和Photorhabdus基因组中发现了新的BGCs,被指定为 plu00736和 plu00747.
- 这些BGCs代表未识别的非核糖体合成酶 (NRPS) 和I型多基合成酶 (T1PKS) 集群.
- 已识别的BGC具有独特的遗传架构,与已知的集群不同,表明新的化合物合成.
结论:
- 基因组挖掘是从微生物中发现BCC和新生物活性化合物的有效策略.
- 已确定的NRPS和T1PKS BGC具有发现新疗法和农业化学品的潜力.
- 需要进一步的研究,包括表达研究和功能表征,以阐明这些新型化合物的生物合成途径和应用.
相关概念视频
03:21Genome Size and the Evolution of New Genes
8.0K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
8.0K
02:55Synthetic Biology
4.8K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Golden rice
Golden rice is a genetically modified...
4.8K
01:20The Central Dogma
21.7K
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
21.7K
02:11Reporter Genes
11.4K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.4K