相关实验视频

Updated: Jul 10, 2025

A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s

A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s

Published on: September 25, 2017

制备金属有机框架@分子印记聚合物,用于提取盐蔬菜中的N-氨胺

Haiyan Chen¹, Xinyu Wang¹, Meijin Lv¹

¹College of Life Science, Yantai University, Yantai 264005, PR China.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

|November 26, 2023

概括

新型混合印制聚合物有效地从制蔬菜中提取N-尼托胺. 这种方法为分析食品中的这些有害化合物提供了灵敏和快速的方法.

科学领域:

材料科学材料科学材料科学
分析化学分析化学
食品安全食品安全

背景情况:

酸氨酸是各种食品中发现的致癌污染物,特别是加工和的蔬菜.
准确检测和量化N-尼托胺对于确保食品安全和监管合规至关重要.

研究的目的:

开发和优化一种用于选择性提取N-尼托胺的新型混合材料.
建立一种敏感且高效的分析方法,用于在制蔬菜中测定N-尼托拉胺.

主要方法:

使用MIL-101和多模拟模板分子制备金属有机框架@分子印记聚合物 (MOF@MIPs).
优化固体相提取 (SPE) 参数用于N-尼托胺丰富.
使用高性能液态染色法-紫外线 (HPLC-UV) 检测对N-尼托拉胺的分析.

主要成果:

开发的MOF@MIP表现出具有高吸附能力 (46.85 mg/g) 和快速平衡 (5 分钟) 的核心外结构.
优化的SPE-HPLC-UV方法显示了0.210μg/g的线性范围,检测极限从20.6到76.1 ng/g.
在盐味蔬菜中的N-尼托拉胺分析中,获得了高峰回收 (66100.5%) 和低相对标准偏差 (0.13.4%).

结论:

新型混合MOF@MIPs在选择性N-尼托胺提取方面有效.
已建立的SPE-HPLC-UV方法是敏感的,高效的,适合直接分析盐蔬菜中的微量N-尼托拉胺.
这种方法有助于改善对致癌污染物的食品安全监测.

关键词:

金属有机框架金属有机框架有分子印记的聚合物.在N-尼托胺中.盐分的蔬菜盐分的蔬菜固体相提取的固体相提取方法

更多相关视频

Author Spotlight: Characterizing Porous Materials for Aiding the Development of Robust Metal-Organic Frameworks with Adsorption Behavior

Author Spotlight: Characterizing Porous Materials for Aiding the Development of Robust Metal-Organic Frameworks with Adsorption Behavior

Published on: March 8, 2024

Preparation of Hydrophobic Metal-Organic Frameworks via Plasma Enhanced Chemical Vapor Deposition of Perfluoroalkanes for the Removal of Ammonia

Preparation of Hydrophobic Metal-Organic Frameworks via Plasma Enhanced Chemical Vapor Deposition of Perfluoroalkanes for the Removal of Ammonia

Published on: October 10, 2013

相关概念视频

2° Amines to N-Nitrosamines: Reaction with NaNO2

2° Amines to N-Nitrosamines: Reaction with NaNO₂

Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.

1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview

1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO₂ Overview

Nitrous acid and nitric acids are two types of acids containing nitrogen, among which nitrous acid is weaker than nitric acid. Nitrous acid with a pKa value of 3.37 ionizes in water to give a nitrite ion and the hydronium ion.
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by...