布鲁塞拉是否会在牛便中分泌出来? - 印度旁遮普邦的证据
V Sharma1, P Kaur2, R S Aulakh1
1Centre for One Health, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India.
Comparative immunology, microbiology and infectious diseases
|November 26, 2023
概括
在印度旁遮普邦的牛便中研究了一种动物传播疾病 - - 牛病. 鉴定了Brucella abortus和Brucella melitensis,突出了动物便传播的风险.
科学领域:
- 兽医微生物学 兽医微生物学
- 动物传播疾病 动物传播疾病
- 公共卫生 公共卫生
背景情况:
- 血病是一种严重的被忽视的动物传播疾病,影响全球动物和人类.
- 这种疾病在印度旁遮普邦的牛群中流行,构成了相当大的公共卫生挑战.
- 关于布鲁塞拉在该地区阳性牛便中的传播存在有限的研究.
研究的目的:
- 为了隔离和识别存在于印度旁遮普邦布鲁塞拉病毒阳性牛便中的布鲁塞拉物种.
- 评估在研究区域通过动物便传播布鲁塞拉病毒的潜在风险.
主要方法:
- 在旁遮普邦卢迪安纳区的350头布鲁塞拉病毒阳性牛身上收集了便样本.
- 隔离涉及选择性和亚格介质,随后进行格拉姆染色和快速幻灯片聚合试验.
- 确认和物种识别使用了属特定的PCR (bcsp31,16s rRNA) 和布鲁斯-拉德多重PCR.
主要成果:
- 从便样本中成功获得了14个布鲁塞拉分离物.
- 所有分离物通过格拉姆染色,凝结试验和PCR证实是布鲁塞拉.
- 鉴定发现了11个Brucella abortus的分离物和3个Brucella melitensis的分离物.
结论:
- 这项研究证实,病毒阳性牛的便可以藏布鲁塞拉菌种,包括Brucella abortus和Brucella melitensis.
- 动物便是布鲁塞洛斯的潜在传播途径,对动物和人类健康都有风险.
- 严格的卫生实践和个人防护设备的使用在处理阳性牛便时至关重要.
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