USP17通过调节NF-κB信号通路通过二基化HDAC2来调节孕前症
Aiping Li1, Ting Wang1, Shasha Zhou2
1Department of Clinical Laboratory, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, 450014, Henan, China.
Placenta
|November 26, 2023
概括
乌比奎丁特异性蛋白酶17 (USP17) 在孕前 (PE) 中是下调的. 恢复USP17通过调节HDAC2和STAT1信号来增强 trofhoblast细胞的增殖和入侵,为PE提供潜在的治疗点.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 生殖医学 生殖医学
背景情况:
- 乌比基因化是一种关键的翻译后修饰,调节重要的细胞过程.
- 无化和双化失调与疾病发病有关,包括孕前 (PE).
- 目前尚不清楚在PE中基因特异蛋白酶17 (USP17) 的作用,它是一种二基因化酶.
研究的目的:
- 调查USP17在孕前症 (PE) 中的作用.
- 阐明USP17在PE病变发生过程中的潜在分子机制.
主要方法:
- 在使用ELISA,Western blot和qRT-PCR的PE患者的血和胎盘组织中量化USP17水平.
- 通过CCK-8,EDU和Transwell测定来评估热囊细胞的增殖,迁移和入侵.
- 通过共免疫沉和西方斑点研究了蛋白质相互作用和信号通路 (HDAC2,STAT1,NF-κB).
主要成果:
- 在PE患者中,USP17表达显著下调.
- USP17过度表达促进了 trofhoblast 细胞的增殖,迁移和入侵.
- USP17对HDAC2进行了双化和稳定,这反过来又增强了STAT1的活动并抑制了NF-κB信号传递.
结论:
- USP17通过增强热囊细胞功能,在孕前中起着保护作用.
- 通过USP17介导的HDAC2二基化是调节热囊细胞行为的一个关键机制.
- USP17代表了孕前的潜在诊断和治疗目标.
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