粘土矿物质上的β-葡萄糖酶:在八基化物合成中的结构和功能
Feng Wang1, Haohao Wang1, Kang Kang1
1Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China.
International journal of biological macromolecules
|November 26, 2023
概括
携带者结合的β-葡萄糖酶 (β-葡萄糖酶) 活性在葡萄糖化合物合成中被阿塔普尔吉特 (Attp) 携带者增强. 分子动力学模拟显示,Attp维持了酶结构和活性,在n-octyl-β-glucoside合成中达到48.3%的产量.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 在酶学中的材料科学.
- 生物物理化学 生物物理化学
背景情况:
- β-葡萄糖酶 (β-葡萄糖酶) 对于水解和合成葡萄糖酶至关重要.
- 载体结合酶可以改善有机溶剂中的糖化物合成,但机制尚不清楚.
- 研究Prunus dulcis β-葡萄糖酶 (PdBg) 活性的粘土矿物载体 (蒙莫里隆石,阿塔普尔吉石,考林石).
研究的目的:
- 为了确定不同粘土矿物载体对β-葡萄糖酶活性和稳定性的影响.
- 为了阐明载体介导增强β-葡萄糖酶活性在糖化物合成中的分子机制.
- 为了优化合成n-octyl-β-glucoside (OGP) 的条件,使用固定β-glucosidase.
主要方法:
- 分子动态 (MD) 模拟用于分析酶基质相互作用和载体效应.
- 测试以测量在蒙特莫里隆石 (Mmt),阿塔普尔吉石 (Attp) 和高石 (Kao) 上固定的β-葡萄糖酶的相对活性.
- 在不同有机溶剂 (t-BA,DMSO,DMF,DME) 中对β-葡萄糖酶的二次结构和形状稳定性的研究.
主要成果:
- 阿塔普尔吉特 (Attp) 固定维持了PdBg活性在81.3%,通过最小影响基质-活性位点距离.
- 蒙莫里隆石 (Mmt) 由于高静电能导致活动最低,而高石 (Kao) 则限制了基板的访问.
- 在溶液中,Attp增强了PdBg的形状稳定性;与其他溶剂不同,三butanol (t-BA) 不会干扰基质结合.
- 使用Attp固定PdBg.g的OGP合成产量达到48.3%.
结论:
- 阿塔普尔吉特是一种优越的载体,可以固定β-葡萄糖酶,从而提高其在有机溶剂中的稳定性和活性.
- MD模拟提供了对载体酶相互作用的分子洞察力,解释了活性差异.
- 通过attp固定的PdBg显示出高效的葡萄糖化合物合成的巨大潜力,特别是OGP.
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