相关实验视频

Updated: Jul 10, 2025

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

Published on: April 21, 2023

缺氧诱导的内皮细胞衍生的外体刺激血管光滑肌肉细胞的增殖和迁移

Qinggen Xiong¹, Fei Lu¹, Xiaoming Xie¹

¹Intervention Department (Vascular Surgery Department) of The Second Affiliated Hospital of Nanchang University, Nanchang, 330008, China.

Biomedical research (Tokyo, Japan)

|November 26, 2023

概括

缺氧促进内皮细胞外体释放,通过TGFBR1通路驱动血管光滑肌细胞的增殖和迁移. 这些外体调解细胞间通信,影响血管健康.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
血管生物学血管生物学

背景情况:

内皮细胞 (ECs) 和血管光滑肌细胞 (VSMCs) 在血管功能中发挥关键作用.
细胞间通信对于维持血管平衡至关重要,特别是在压力条件下,如缺氧.
外体是细胞间通信的关键媒介,但它们在缺氧诱导的VSMC功能障碍中的作用尚未完全理解.

研究的目的:

在低氧条件下,研究从内皮细胞 (ECs) 衍生出来的外体细胞在调解与血管光滑肌细胞 (VSMCs) 的通信中的作用.
探索EC衍生外体对VSMC增殖和迁移的影响机制.
确定转化生长因子-β受体1 (TGFBR1) 途径在这个过程中的参与.

主要方法:

在正常和缺氧条件下,人体VSMC和EC的体外培养.
从ECs中分离和表征外体.
细胞功能测试用于评估VSMC的增殖和迁移.
西部斑点分析以评估TGFBR1通路相关的蛋白质表达.
在EC中抑制TGFBR1,以评估其在外体介导效应中的作用.

主要成果:

缺氧改变了ECs的外体分泌.
由ECs衍生的外体细胞 (ECs-Exos) 在低氧条件下显著增加了VSMC的增殖和迁移.
在VSMC中,ECs-Exos对TGFBR1通路相关蛋白质的表达进行了上调.
在EC中抑制TGFBR1逆转了ECs-Exos在低氧下对VSMC增殖和迁移的促进作用.

结论:

缺氧诱导EC释放外体,促进VSMC异常增殖和迁移.
来自ECs的外体提供TGFBR1,调解细胞间通信,并导致VSMC功能障碍.
向EC衍生的外体或TGFBR1通路可能为缺氧相关的血管疾病提供治疗策略.

更多相关视频

A Cell Culture Model of Resistance Arteries

A Cell Culture Model of Resistance Arteries

Published on: September 8, 2017

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Paracrine Signaling

Paracrine Signaling

Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...