在菲律宾卡维特山羊中检测Trypanosoma evansi的分子检测和内部转录的间隔-1序列多样性
Adrian Miki C Macalanda1,2, Eloiza May S Galon1,2, Vernadyn A Morillo3
1Department of Immunopathology and Microbiology, College of Veterinary Medicine and Biomedical Sciences, Cavite State University, Cavite, Philippines.
The Journal of veterinary medical science
|November 26, 2023
概括
菲律宾卡维特 (Cavite) 的45%的山羊中发现了一种影响牲畜的疾病Surra. 这项研究强调了山羊中Trypanosoma evansi的流行情况,并表明了物种间的潜在传播.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 分子流行病学分子流行病学
- 畜牧业的健康 畜牧业的健康
背景情况:
- 羊的生产对于生计和粮食安全至关重要.
- 羊可以作为苏拉的水库,影响畜牧业生产.
- 之前的研究报告说,塞布的山羊中surra的流行率较低.
研究的目的:
- 为了选菲律宾卡维特的自由流浪山羊,寻找它们的外表.
- 为了确定山羊中Trypanosoma evansi的流行率和家族遗传特征.
- 为了调查潜在的跨物种传播的超级.
主要方法:
- 使用PCR和两套初始剂组对42只自由流浪的山羊进行查.
- 使用Trypanosoma brucei微卫星染色体原始物的初始检测.
- 使用内部转录间隔器1 (ITS-1) 区域进行的家族遗传学分析.
主要成果:
- 45%的山羊 (19/42) 检测出surra阳性,比之前报告的更高.
- 男性的感染率 (56%) 比女性 (42%) 高.
- 年轻山羊 (≤1岁) 显示100%的传染性,而成年人 (>1岁) 的感染率为43%.
结论:
- 在菲律宾卡维特的山羊中,Trypanosoma evansi.的流行率很高.
- 年龄和性别对山羊的感染易感性有影响.
- 遗传学分析表明,T. evansi. 的潜在物种间传播.
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