一个合理设计的二氧化硫及其衍生物的光探针:在食品分析和生物成像中的应用
Yuyu Fang1,2, Dongbin Zheng3, Tingrui Zhang3
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China. yyfang@cdutcm.edu.cn.
Analytical and bioanalytical chemistry
|November 26, 2023
概括
一个新的光探测器,PIB,使得二氧化硫 (SO2) 和其衍生物的敏感和选择性检测. 这个探测器可视化食物,细胞和斑马鱼中的SO2,揭示其与细胞亡的联系.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 细胞生物学 细胞生物学
背景情况:
- 二氧化硫 (SO2) 和其衍生物用于食品保存,并充当信号分子.
- 过度暴露于SO2可能会对健康产生不利影响,需要有效的监测方法.
- 目前用于检测SO2的方法在灵敏度,选择性或在生物系统中的应用性方面是有限的.
研究的目的:
- 开发一种针对线粒体的新型光探针,用于对SO2及其衍生物的敏感和定量检测.
- 评估探测器在各种矩阵中的性能,包括食物,细胞和生物.
- 研究内源性SO2在细胞过程中的作用,特别是细胞亡.
主要方法:
- 一个针对线粒体的光探针 (PIB) 的合成和特征.
- 以高灵敏度和选择性检测SO2及其衍生物的比度测量.
- 在食品样本 (糖,葡萄酒) 中测量SO2量化的探针的应用.
- 在细胞和斑马鱼中的SO2的体外和体外成像.
- 研究SO2在亡中的作用及其在小鼠器官中的代谢局部化.
主要成果:
- PIB探测器表现出极好的灵敏度 (DL = 15.9 nM) 和快速响应时间 (200秒).
- 探测器在广泛的pH范围 (6.510.0) 中表现出高选择性和稳定性.
- 在实物食品样本中,PIB成功量化了SO2,并在细胞和斑马鱼线粒体中成像了SO2.
- 确立了内源性SO2和亡之间的显著相关性.
- 在小鼠器官中实现了SO2的局部代谢定位.
结论:
- 开发的PIB探头是SO2及其衍生物的比度检测和成像的一个有价值的工具.
- PIB促进了SO2在生物系统中的生理和病理作用的研究.
- 探测器的线粒体准能力和灵敏度为探索与SO2相关的细胞机制,包括细胞亡,开辟了道路.
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