来自巴厘岛Tulamben的海绵 Aaptos suberitoides的原生生物群落和aptamines的概况
Ni Kadek Dita Cahyani1, Noer Kasanah2, Dewi Sri Kurnia3
1Biology Department, Faculty of Science and Mathematics, Diponegoro University, Semarang, Central Java, Indonesia.
Marine biotechnology (New York, N.Y.)
|November 26, 2023
概括
像Aaptos suberitoides这样的海洋海绵是各种微生物的宿主,它们产生生物活性化合物. 这项研究揭示了印尼A. suberitoides海绵中的 prokaryotic 社区和 aaptamine 代谢产物.
科学领域:
- 海洋微生物学 海洋微生物学
- 自然产品化学 自然产品化学
- 生物活性化合物 生物活性化合物
背景情况:
- 海洋海绵有各种微生物群落,对于生产生物活性化合物至关重要.
- 微生物显著影响海绵衍生的天然产品的化学多样性.
- 印度尼西亚海绵Aaptos suberitoides是新生物活性代谢物的潜在来源.
研究的目的:
- 描述与Aaptos suberitoides相关的 prokaryotic 社区组成.
- 为了识别和分析A. suberitoides样本中的阿帕坦胺代谢物.
- 建立对印尼A. suberitoides微生物和化学特征的基本理解.
主要方法:
- 使用DNA条形编码 (细胞氧化酶子单元I基因) 进行海绵识别.
- 通过16S rRNA基因放大和测序 (V4区域) 来构建 prokaryotic 社区概况.
- 使用液体染色学-质谱学 (LC-MS) 和脱复制用于阿帕坦胺识别的代谢物分析.
主要成果:
- 这些海绵样本被证实是Aaptos suberitoides.
- 主导的 prokaryotic 分类包括 蛋白质细菌, , Actinobacteria 和 酸性细菌.
- 在大多数样本中检测到阿巴胺和异阿巴胺; 一个样本产生了阿巴胺类似物.
结论:
- 这项研究提供了第一份关于印尼巴厘岛热带海洋海绵A. suberitoides的 prokaryotic 社区和 aaptamine 资料的报告.
- 这些发现突显了微生物共生体与海洋海绵中特定代谢物的产生之间的联系.
- 进一步的研究可以探索已识别的aaptamines及其来自A. suberitoides的衍生物的生物活性.
更多相关视频
08:09An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Published on: September 15, 2015
8.8K
09:49Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
Published on: October 31, 2019
22.4K
相关概念视频
The Colonization of Land
Changes in the environment of the early Earth drove the evolution of organisms. As prokaryotic organisms in the oceans began to photosynthesize, they produced oxygen. Eventually, oxygen saturated the oceans and entered the air, resulting in an increase in atmospheric oxygen concentration, known as the oxygen revolution approximately 2.3 billion years ago. Therefore, organisms that could use oxygen for cellular respiration had an advantage. More than 1.5 years ago, eukaryotic cells and...
Sulfur Assimilation
Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to become...
Diversity of Protists I
Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
Microbial Mats
Microbial communities forming biofilms and mats represent complex, spatially structured ecosystems where metabolic processes are stratified according to light, oxygen, and nutrient gradients. Biofilms are initial colonization stages, only a few millimeters thick, while mature microbial mats can reach centimeter-scale thickness and display intricate vertical organization. Their structural and functional heterogeneity allows microorganisms to occupy distinct ecological niches within a few...
Deep Sea Microbial Ecology
The deep ocean and its underlying sediments represent vast, largely unexplored microbial habitats that extend far beyond the sunlit photic zone. The photic (euphotic) zone typically spans the upper ~100–200 meters of pelagic waters in the open ocean, but its depth varies geographically and seasonally, where sufficient light supports photosynthetic life. Below this lies the deep sea, spanning roughly 1000–6000 meters (bathypelagic to abyssal zones), with deeper hadal trenches extending beyond...
iChip
The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
