在体外实验中验证阴道微生物组代理的实验,即生物模拟乳杆菌主导的阴道培养
Abir Zahra1, Ramkumar Menon2, Giovana Fernanda Cosi Bento2,3
1School of Medicine, The University of Texas Medical Branch at Galveston, Galveston, Texas, USA.
American journal of reproductive immunology (New York, N.Y. : 1989)
|November 27, 2023
概括
来自阴道微生物群的微生物代谢物可以在体外准确地模仿其健康的抗炎作用. 这项研究表明,来自健康的阴道微生物群的代谢物抑制炎症,与来自细菌性阴道炎的代谢物不同.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
背景情况:
- 阴道微生物群,由乳酸菌种占主导地位,对于维持平衡和预防感染至关重要.
- 现有的体外模型难以复制复杂的阴道微环境,限制了对其功能的研究.
- 微生物代谢物被探索为潜在的代理物,以代表阴道微生物群在细胞培养中的影响.
研究的目的:
- 在体外模型中评估微生物代谢物作为阴道微生物群的代理物的实用性.
- 研究来自健康的代谢物与与细菌性阴道炎相关的微生物组的抗炎潜力.
主要方法:
- 人类永生阴道上皮细胞 (VECs) 在跨井培养,并与健康或细菌性阴道病 (BV) 微生物群结合.
- 对超级生物的代谢分析使用质谱和英才途径分析 (IPA) 确定了关键代谢物.
- 无细菌代谢物被添加到VEC培养物中,以评估它们对细胞活力,形态和细胞因子产生的影响.
主要成果:
- 来自健康的阴道微生物组的代谢物含有与抗炎和代谢信号相关的脂肪酸,核酸和糖酸.
- 这些健康的代谢物诱导了VEC的抗炎环境,增加IL-10并抑制IL-6,IL-8和TNFα.
- 来自BV相关微生物组的代谢物诱导了促炎环境,提高了IL-6,IL-8和TNFα水平.
结论:
- 阴道上皮细胞透孔系统有效支持相关微生物代谢物的产生.
- 微生物代谢物可以在体外准确地重现健康的阴道微生物群的抗炎环境.
- 这种基于代谢物的方法提高了用于研究阴道健康和疾病的体外模型的准确性和实用性.
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