在一个可调节的闭开数字微流体系统上进行滴滴操纵,使用不对称的EWOD
Jingsong Xu1, Xingcheng Wang1, Qingyuan Huang1
1School of Information Science and Engineering, Lanzhou University, No. 222 Tianshui South Road, Lanzhou 730000, China. xdhe@lzu.edu.cn.
Lab on a chip
|November 27, 2023
概括
一个新的数字微流体 (DMF) 平台结合了封闭和开放的系统,使用修改的表面进行可扩展的滴滴操纵. 这项创新使得有效的蛋白质检测和实时生物样本处理成为可能.
科学领域:
- 微流体学 微流体学
- 表面科学是一门学科.
- 生物技术是生物技术.
背景情况:
- 封闭式开放式数字微流体 (DMF) 系统整合了这两种设计的优势.
- 由于封闭和开放区域的电极结构不同,当前的系统面临可扩展性问题.
研究的目的:
- 开发一个可调节,可扩展和具有成本效益的封闭式开放式DMF平台.
- 调查闭开边界的滴滴操纵,并优化平台参数.
- 为了证明平台在生物样本处理和检测中的实用性.
主要方法:
- 使用改造的滑动液体注入多孔表面 (SLIPS) 与不对称的电湿对介电 (AEWOD).
- 使用一致的印刷电路板 (PCB) 电极阵列和浮动电位顶板.
- 研究了电压,板间距,开关间隔和驾驶策略对滴滴操纵的影响.
- 分析了顶板几何形状和边缘滴滴运动的斜角.
主要成果:
- 实现了低成本,可扩展的封闭开放的DMF系统,具有一致的电极结构.
- 在封闭区域中确定了最佳的板间距 (340-510微米).
- 确定一个薄的顶部板与一个~4°的曲方便滴滴运动在边界.
- 成功进行了蛋白质染色,并开发了一个用于检测蛋白质度的智能手机应用程序.
结论:
- 开发的可调节的封闭开放的DMF平台克服了可扩展性的挑战.
- 该平台可实现精确的滴滴控制和高效的生物分析.
- 该系统为实时生物样本处理和检测应用提供了巨大的潜力.
相关概念视频
01:16Control Volume and System Representations
Two key frameworks are employed to analyze mass, energy, and momentum transfer: the control volume approach and the system approach. These frameworks offer different perspectives, depending on whether the focus is on a specific region in space (control volume approach) or a defined mass of fluid (system approach).
The control volume approach considers a stationary region in space through which fluid flows. This region is bounded by a control surface. For instance, in the case of water flowing...
The control volume approach considers a stationary region in space through which fluid flows. This region is bounded by a control surface. For instance, in the case of water flowing...
01:30Typical Model Studies
Fluid mechanics model studies often utilize scaled-down systems to predict fluid behavior in full-scale environments, such as river flows, dam spillways, and structures interacting with open surfaces. Maintaining Froude number similarity in river models is crucial, as it replicates surface flow features like wave patterns and velocities.
01:21Design Example: Creating a Hydraulic Model of a Dam Spillway
Scaled hydraulic models of dam spillways provide a practical way to replicate and study the intricate flow dynamics of these structures. Often built to a 1:15 ratio, these models allow for observing critical water behavior, such as velocity distribution, flow patterns, and energy dissipation.