探索Bacillus amyloliquefaciens菌株OM81用于使用橄厂废水生产聚酸酸盐 (PHA) 生物塑料
Samar Bacha1, Fatma Arous2, Emna Chouikh1
1University of Carthage, INSAT, Centre Urbain Nord, B.P. 676, 1080 Tunis, Tunisia.
3 Biotech
|November 27, 2023
概括
百菌氨基面菌株OM81有效地使用橄厂废水生产多酸酸盐 (PHAs). 这种可持续的方法减少了污染和生物塑料生产成本.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 环境科学 环境科学
背景情况:
- 橄油厂废水 (OMW) 是一个污染的副产品.
- 生物塑料,如聚基酸盐 (PHAs),为传统塑料提供可持续的替代品.
- 开发具有成本效益的PHA生产方法对于广泛采用至关重要.
研究的目的:
- 评估OMW中的细菌菌株对PHA积累的情况.
- 调查OMW作为PHA生产的碳来源的潜力.
- 为了描述产生的生物聚合物.
主要方法:
- 从OMW中分离和选细菌菌株.
- 在合成和基于OMW的介质上培养*Bacillus amyloliquefaciens* OM81.
- 通过苏丹黑色染色来量化PHA.
- 使用FTIR,DSC和TGA进行生物聚合物表征.
主要成果:
- *Bacillus amyloliquefaciens* OM81表明PHA的积累是显著的.
- 最大PHA产量为0.14g/L (30.2%重量/重量). 干燥生物质) 在合成介质中实现.
- OMW支持细菌生长,在50%的OMW度下观察到最大的生长.
- FTIR分析证实了PHB碳基的存在.
结论:
- *Bacillus amyloliquefaciens* OM81是PHA生物生产的一个有希望的候选者.
- 使用OMW作为碳来源可以降低生物塑料生产成本并减轻污染.
- 这项研究强调了生物塑料制造的可持续方法.
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