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细胞壁局部化的Bt蛋白赋予了大米对虫害虫的高抵抗力

Hua Li^1,2, Lihua Deng¹, Lvshui Weng¹

¹Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.

Pest management science

|November 27, 2023

概括

工程化Bacillus thuringiensis (Bt) 融合蛋白在米的细胞壁上,为类虫害提供了高抵抗力. 这一策略尽量减少了意外的影响,保持了改良作物的正常农学特征.

科学领域:

农业科学农业科学
生物技术是生物技术.
分子生物学分子生物学

背景情况:

商业化的Bt作物会在细胞内积累Bt蛋白,从而可能造成意想不到的影响.
开发了一种新的方法,可以将Bt蛋白分泌并固定在细胞壁上,从而最大限度地减少细胞内相互作用.

研究的目的:

创建一个融合基因 (SP-Cry1Ca-CBM11),用于分泌和定Bt蛋白 (Cry1Ca) 在米细胞壁上.
评估改造Bt大米对虫害的有效性和安全性.

主要方法:

使用细胞外分泌信号和碳水化合物结合模块11序列构建了一个融合基因SP-Cry1Ca-CBM11.
通过细胞壁固的构成性促进体,将融合基因引入大米中.
在烟草中进行过渡表达,并生成用于分析的转基因大米线.

主要成果:

短暂的表达证实了融合蛋白对细胞壁的定.
转基因大米品种表现出高Cry1Ca含量,特别是在叶子中 (高达17.55μg g-1).
对粉红色茎,条纹茎和大米叶滚筒的耐药性很高,修正后的死亡率从83.32%到100%不等.

结论:

细胞壁固的融合Bt蛋白有效地赋予大米中对类虫害的高耐药性.
转基因大米表现出正常的农学特征和抗草甘耐药性,表明没有意外影响.
这一战略为开发更安全,更有效的Bt作物提供了一个有前途的方法.

关键词:

虫虫害害虫害虫害害虫害虫害害虫害害虫害虫害虫害害虫害虫害害害虫害虫害虫害虫害害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害虫害细胞壁上的细胞壁.的交叉电阻.方向积累的方向积累是指向性的.更高的折叠更高的折叠.草甘是一种草甘.杀虫剂蛋白是一种杀虫剂蛋白.电阻的电阻是指电阻的电阻

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