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一个YTHDF-PABP相互作用是植物中A介导的有机生成所必需的

Mathias Due Tankmar¹, Marlene Reichel¹, Laura Arribas-Hernández¹

¹Department of Biology, University of Copenhagen, Copenhagen, Denmark.

|November 27, 2023

概括

ECT2蛋白的本质上有障碍的区域对于植物器官生成至关重要. 一个特定的区域结合多A结合蛋白,对叶子形成和细胞分裂至关重要.

科学领域:

植物分子生物学植物分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
发展生物学发展生物学

背景情况:

N6-甲基氨酸 (m6A) 是一种关键的mRNA修饰,可以调节真核生物的基因表达.
YT521-B同质 (YTH) 域蛋白质识别了m6A的修改.
在YTH蛋白中,内在无序区域 (IDR) 参与了相分离和蛋白相互作用,但它们的功能基本上是未知的.

研究的目的:

阐明Arabidopsis thalianaECT2蛋白刺激器官生成的分子机制.
在 ECT2 的内在无序区域 (IDR) 中识别特定的功能元素,这对于其在植物发育中的作用至关重要.

主要方法:

研究了 ECT2 的 N-终端内在无序区域 (IDR) 在叶子形成中的作用.
确定了特定的短IDR元素,这些元素对ECT2介导的器官生成至关重要.
使用蛋白质-蛋白质相互作用测试来确定ECT2.2的结合伙伴.

主要成果:

ECT2的长N端IDR对于刺激叶子形成至关重要.
在ECT2中,两个短IDR元素是其在有机生成中的功能所必需的.
一个含有保存型氨酸丰富基因的19氨基酸区域介导了ECT2与多A结合蛋白 (PAB2,PAB4,PAB8) 的结合.
过度表达PAB4部分地挽救了ect2ect3ect4突变的器官发生缺陷.

结论:

ECT2的IDRs通过与多A结合蛋白相互作用,在调节器官发生过程中发挥关键作用.
针对mRNA的ECT2和PAB蛋白之间的相互作用对于刺激细胞分裂至关重要.
这种相互作用可能会在发育中的植物组织中克服PAB度限制,突出显示植物发育中的新型调节机制.

关键词:

阿拉比多普西斯 (Arabidopsis) 是一种植物.欧洲2 欧洲2 欧洲2 这是一个IDR IDR.在PABP中,PABP是PABP.m6A 一个很好的.

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