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在苛刻的环境中无标签的单个纳米粒子识别和表征,包括传染性新兴病毒

Minh-Chau Nguyen¹, Peter Bonnaud¹, Rayane Dibsy²

¹UMR 7252, CNRS, XLIM, Université de Limoges, Limoges, F-87000, France.

Small (Weinheim an der Bergstrasse, Germany)

|November 27, 2023

概括

瑞托夫纳米粒子识别显微镜 (RYMINI) 提供无标签的,非侵入性的纳米粒子分析. 这种方法可以准确地识别和表征各种纳米粒子,包括病毒,用于各种应用的溶液.

科学领域:

光学物理学的光学物理.
纳米技术纳米技术
生物医学工程生物医学工程

背景情况:

准确识别和描述未知的纳米粒子对于医学,工业和环境监测至关重要.
现有的方法通常需要染色或具有破坏性,限制了它们在敏感环境中的适用性.

研究的目的:

为单个纳米粒子识别和表征引入一种新,无染色,非侵入性和非破坏性的光学方法.
证明系统在苛刻环境中的能力,例如BSL-3实验室.

主要方法:

开发和实施RYtov微镜用于纳米粒子识别 (RYMINI).
整合全息无标签3D跟踪与高灵敏度定量相位成像.
在溶液中的单个纳米颗粒的计量表征,包括病毒和细胞外囊泡.

主要成果:

在没有事先的知识的情况下,RYMINI可以准确地确定单个纳米粒子的性质,度,大小,复杂折射率和质量.
在分辨介电,金属和生物纳米粒子方面达到>90%的准确性.
对于金属和介电纳米粒子的类内化学测定,证明了80%的准确性,对于生物纳米粒子类型的测定,准确率为50-70%.

结论:

瑞米尼是用于在具有挑战性的环境中进行纳米粒子分析的强大而多功能工具.
该技术使纳米物体的全面,无标签的表征成为可能,进步了病毒学和材料科学等领域.
在单个粒子层面上,RYMINI在纳米颗粒的计量表征方面取得了重大进展.

关键词:

传染性病毒成像成像技术没有标签的单个纳米粒子识别识别.定量阶段显微镜学的定量阶段显微镜.单个纳米物体计量学

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