一个用于miRNA传感和基因调节的放射性基因切换系统
Chuanxian Zhang1, Beilei Zhang2, Chu Tang3
1School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an, 710061, China.
Small methods
|November 27, 2023
概括
研究人员开发了一种新的基因开关系统 (Gal-miR),以可视化和控制微RNA (miRNA) 活动. 这种系统可以实现精确的基因调节,并为研究生物过程和疾病中的miRNA动态提供了强大的工具.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 生物技术是生物技术.
背景情况:
- 微RNA (miRNA) 是小型非编码RNA,在转录后调节基因表达.
- 目前的合成RNA开关通常依赖于连接体分子,限制了特异性和效率.
- 存在需要精确的,配体独立的miRNA可视化和基因调节系统.
研究的目的:
- 描述一种基于Gal4BD-Rluc的新型融合转录抑制器基因切换系统 (Gal-miR),用于miRNA可视化和基因调节.
- 为了证明Gal-miR系统能够定量反映miRNA活动的能力.
- 通过控制基因表达来展示该系统在治疗应用中的潜力.
主要方法:
- 使用Gal4BD-Rluc融合蛋白的Gal-miR基因开关系统的开发.
- 一个由内源性miRNA机器控制的光酶记者基因的整合.
- 该系统的应用用于在肌原分化过程中监测miRNA-206活动.
- 通过将记者基因替换为梅利丁功能基因来抑制癌细胞功能来修改系统.
主要成果:
- 该Gal-miR系统成功地将miRNA介导的基因沉默转化为比度生物发光信号.
- 在肌原分化过程中实现了miRNA-206活动的定量反射.
- 修改后的基因切换系统有效地抑制了乳腺癌细胞的活力,迁移和入侵.
- 通过miRNA响应的方式精确控制了转基因表达.
结论:
- 该Gal-miR系统提供了一个强大的模块化遗传设计,用于miRNA可视化和基因调节.
- 该系统允许精确控制转基因表达,以应对特定的miRNAs.
- -miR系统为可视化miRNA动态和探索治疗策略提供了一个有价值的工具.
更多相关视频
相关概念视频
Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
MicroRNAs
21.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.3K
Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
RNA Interference
26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.1K
siRNA - Small Interfering RNAs
16.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.8K
piRNA - Piwi-interacting RNAs
6.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K


