甲状腺激素增加胎儿羊心肌细胞脂肪酸的使用
Natasha Chattergoon1, Samantha Louey1, Sonnet S Jonker1
1Center for Developmental Health, Knight Cardiovascular Institute, Oregon Health & Science University, Portland, Oregon, USA.
Physiological reports
|November 27, 2023
概括
甲状腺激素 (T3) 通过增加脂肪酸代谢,促进胎儿心肌细胞的成熟. 然而,长时间接触棕酸 (PA) 可能会阻碍这种氧化能力,影响心脏发育.
科学领域:
- 心脏病学 心脏病学
- 代谢研究研究 代谢研究
- 发展生物学 发展生物学
背景情况:
- 心脏代谢基质偏好在分娩时发生变化,偏好脂肪酸而不是碳水化合物.
- 甲状腺激素 (T3) 在胎儿发育和代谢调节中起着至关重要的作用.
研究的目的:
- 研究甲状腺激素 (T3) 和棕酸 (PA) 对胎儿心肌细胞氧化代谢的影响.
- 为了确定T3和PA是否刺激胎儿心肌细胞氧化代谢能力.
主要方法:
- 甲状腺激素 (T3) 被注入到胎儿的羊身上.
- 用BODIPY标记的脂肪酸评估心肌细胞对脂质的吸收和滴滴的形成.
- 心肌基因表达通过PCR分析.
- 在体外暴露于T3和PA后,使用海马生物分析仪测量了心肌细胞的氧气消耗率.
主要成果:
- 在子宫内,T3暴露会使长链脂肪酸滴滴在心肌细胞中的积累增加42%.
- 参与脂肪酸代谢和氧化 (CD36,CPT1A,CPT1B,LCAD,VLCAD,HADH,IDH,PDK4) 的关键基因的T3上调节的心肌mRNA表达.
- 在体外T3暴露增加了心肌细胞的氧气消耗,特别是当PA急性供应时;然而,长时间的PA暴露取消了T3刺激的氧气消耗.
结论:
- 甲状腺激素 (T3) 有助于胎儿心肌细胞的代谢成熟,增强其脂肪酸氧化能力.
- 胎儿心肌细胞长时间暴露在棕酸 (PA) 中可能会损害氧化能力,可能会影响发育过程中的心脏功能.
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