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在慢性鼻炎中,持续性黄金葡萄球菌的宿主内部进化格局和病变适应
Ghais Houtak1,2, George Bouras1,2, Roshan Nepal1,2
1Adelaide Medical School, Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, Australia.
Microbial genomics
|November 27, 2023
概括
黄金葡萄球菌可以在慢性鼻炎 (CRS) 患者中持续存在,发展出更高的抗生素耐受性和生物膜形成. 这些持续性感染显示出特定的遗传变化,表明在鼻环境中存在一种病变适应过程.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 慢性鼻炎 (CRS) 的特点是持续的鼻炎症.
- 黄金葡萄球菌生物膜经常与CRS有关,导致复发性感染.
- 了解S. aureus持久性机制对于有效的CRS管理至关重要.
研究的目的:
- 确定CRS患者中黄金葡萄球菌持久性和病变适应率.
- 分析纵向采集的S. aureus分离物的基因组相关性和抗生素耐药性/耐受性.
- 在持久的金黄色菌株中调查突变变化和生物膜适应.
主要方法:
- 在至少6个月内从34名CRS患者收集了68个S. aureus配对分离物 (34对).
- 在48小时的生物膜中评估了抗生素耐受性.
- 利用混合测序来获得高质量的基因组组,并分析单核酸变异 (SNV),结构变异和等离子体动力学.
主要成果:
- 41%的S. aureus孤立对在CRS患者中表现为持久性.
- 持久隔离物在粘附基因和移动遗传元素中表现出特定突变,等离子体拷贝数量增加.
- 持久菌株显著增加了对抗生素的生物膜耐受性和增强的生物膜生物量,表明了病变适应.
结论:
- 黄金菌可以在CRS患者中持续存在,经历遗传和适应性变化.
- 持续孤立的突变事件有利于宿主粘附和移动遗传元素动态.
- 在持久性黄金色杆菌中增加的生物膜生物量和抗生素耐受性表明一种病变适应过程有助于CRS慢性.
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