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用2D模板匹配的诱重建用于高分辨率的体外和体内结构确定,没有模板偏差

Bronwyn A Lucas^1,2,3, Benjamin A Himes^1,4, Nikolaus Grigorieff^1,4

¹RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, United States.

|November 27, 2023

概括

我们展示了冷电子显微镜 (cryo-EM) 中的2D模板匹配 (2DTM) 能够实现精确的定位和高分辨率的3D重建. 这种方法最大限度地减少模板偏差,允许准确可视化初始模板中不包含的分子特征.

科学领域:

结构生物学结构生物学
生物物理学的生物物理.
生物化学生物化学

背景情况:

2D模板匹配 (2DTM) 精确地定位了冷电子显微镜 (cryo-EM) 图像中的宏分子复合体.
之前的工作确立了2DTM在杂的蜂环境中的有效性.

研究的目的:

从2DTM局部化的粒子研究生成高分辨率的3D重建.
在3D重建中评估和最小化模板偏差.
为模板偏差开发一个定量指标.

主要方法:

使用二维模板匹配 (2DTM) 定位宏分子复合物.
平均局部粒子生成3D冷EM重建.
评估条件以尽量减少模板偏差,同时保持本地化准确性.

主要成果:

高分辨率的3D重建可以从2DTM局部化的粒子生成.
模板中缺少的分子特征可以在高分辨率下重建.
开发了一个量化指标来评估模板偏差.

结论:

2DTM可提供高分辨率的3D冷EM重建,模板偏差最小化.
从高分辨率模板获得的解释重建的开发的度量辅助器.
这种方法提高了冷EM中结构特征分析的可靠性.

关键词:

三维结构的3D结构.美国大肠杆菌 (E. coli).它们中的一种是S. cerevisiae.细胞生物学细胞生物学低温电磁波冷却器 (Cryo-EM) 是一个非常好的方法.电子显微镜的电子显微镜图像分析图像分析在现场 (in situ) 进行.分子生物物理学分子生物物理学核糖体的核糖体是指核糖体中的核糖体.结构生物学结构生物学

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