20基和相关基因是否是对脂质改变污染物的次致命暴露的潜在生物标志物?
Hugo Alarie1, Nadia Côté2, Luc Gaudreau2
1Department of Chemistry, Université de Sherbrooke, Sherbrooke, QC, J1K 2R1, Canada.
Environmental science and pollution research international
|November 27, 2023
概括
这项研究发现,大方的20-二 (20E) 水平和相关基因表达不是对利摩尔或降脂药物的次致命暴露敏感的生物标志物. 生殖受到了费纳里摩尔的影响,但分子终点没有显著变化.
科学领域:
- 环境毒理学环境毒理学
- 在无脊椎动物中生态类固醇代谢.
- 在水生生物体中发现生物标志物.
背景情况:
- 20-二 (20E) 是大的主要变激素.
- 确定污染物暴露的可靠生物标志物对于环境监测至关重要.
- 了解ecdysteroid代谢有助于评估水生无脊椎动物的内分泌干扰.
研究的目的:
- 为了确定20E的基线水平和Daphnia magna.中相关的基因表达.
- 评价利摩尔和米布罗西尔/克洛纤维酸混合物对大夫尼亚大菌的作用.
- 评估20E和特定基因转录作为亚致命污染物暴露的生物标志物的潜力.
主要方法:
- 大夫尼亚大被暴露于费纳里莫尔和米布罗西尔/克洛纤维酸混合物,持续21天.
- 测量的终点包括四个与ecdysteroid相关的基因的死亡率,繁殖率,20E量化和转录水平.
- 在暴露研究之前评估了20E和基因表达的基线水平.
主要成果:
- 利莫尔暴露显著降低了大夫尼亚大的繁殖在565μg/L.
- 暴露于费纳里摩尔或降脂药物混合物后,没有观察到20E水平或测试基因转录的显著变化.
- 基因表达和20E水平的基线变化很高,可能是由于发育阶段的差异.
结论:
- 目前的20E水平和选择的基因转录水平不是对利或大夫尼亚大中测试的脂质改变化合物的亚致命暴露的敏感生物标志物.
- 需要更多的研究,包括多个时间点和转录组/代谢组方法,以确定对亚致命暴露的有效生物标志物.
- 生殖产出仍然是暴露在某些环境污染物中的大夫尼玛格纳压力的敏感指标.
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