在玻璃体内,在斑马鱼和梅达卡的室温下存储功能性精子
Kazumasa Takemoto1, Toshiya Nishimura2, Toshihiro Kawasaki1,3
1Department of Genetics, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Mishima, Japan.
Zebrafish
|November 27, 2023
概括
研究人员开发了一种新的储存介质,在室温下将斑马鱼精子的存活时间延长到28天,而梅达卡精子的存活时间则延长到7天,从而实现了低成本的运输和生育研究.
科学领域:
- 水生生物学 水生生物学
- 生殖科学 生殖科学
- 精子冷保存 精子冷保存
背景情况:
- 斑马鱼和梅达卡等远程生物中的精子寿命通常在隔离状态下很短.
- 内部肥料表明长期储存精子,这表明在最佳条件下外部肥料的潜力.
- 目前对精子存活的最佳条件的理解是有限的.
研究的目的:
- 开发和验证一种存储介质,以延长斑马鱼和鱼的精子活力.
- 评估储存在发育媒介中的精子的生育能力.
- 为了实现经济高效的精子运输,并促进对精子运动和生育因子的研究.
主要方法:
- 斑马鱼和梅达卡的精子被存储在一个以L-15为基础的介质中,并补充了牛血清白蛋白,胎儿牛血清,葡萄糖和乳酸.
- 储存是在室温下进行的.
- 通过受精卵和观察胚胎发育到肥沃的成年人来评估生育能力.
主要成果:
- 斑马鱼的精子在室温的储存介质中保持了28天的生存能力和生育能力.
- 在相同的条件下,梅达卡精子在7天内保持活力和生育能力.
- 受精的胚胎发展成正常的,肥沃的成年鱼,证实了精子的质量.
结论:
- 开发的存储介质在室温下显著延长了斑马鱼和梅达卡精子的生存能力和生育能力.
- 这种方法支持延长精子存活时间,与生理条件相比,至少4周 (斑马鱼) 和1周 (梅达卡).
- 这些发现促进了低成本的精子运输,并促进了这些物种对精子运动和生育能力的研究.
相关概念视频
01:41Spermatogenesis
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
01:20Preparation of Samples for Electron Microscopy
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
01:15Sperm Transport
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
01:22Sperm Structure and Semen Composition
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...