相关实验视频

Updated: Jul 9, 2025

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

表观遗传开关重塑了表皮原生细胞的特征,并驱动了hidradenitis suppurativa炎症的炎症病变发生

Lin Jin^1,2,3, Yunjia Chen⁴, Suhail Muzaffar^2,3

¹Center for Epigenomics and Translational Research in Inflammatory Skin Diseases, Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL 35294.

Proceedings of the National Academy of Sciences of the United States of America

|November 27, 2023

概括

补腺炎 (HS) 涉及皮肤原生细胞变得致病,导致炎症. 表观遗传变化和特定的基因表达驱动HS,提供新的治疗点.

科学领域:

皮肤病学皮肤病学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

补腺炎 (HS) 是一种慢性炎症性皮肤疾病,其机制尚不清楚.
表皮细胞功能障碍与HS的发病有关,但特定的细胞和分子驱动因素仍然不清楚.

研究的目的:

为了研究上皮原生细胞在HS中的作用.
阐明驱动HS病变的分子和表观遗传机制.
为了确定HS的潜在治疗点.

主要方法:

从HS和健康皮肤上皮质干细胞/原生细胞中对基因表达和表观基因组数据集的比较分析.
细胞分化轨迹重建和CellChat建模以确定HS特异性细胞群.
编辑CRISPR/Cas9基因和制药抑制以准已识别的途径.

主要成果:

在HS的基底原生细胞中,表现出改变的血统限制,形成致病性角质细胞克隆.
HS角质细胞表现出与细胞循环,DNA修复,细胞粘附和染色质重塑相关的独特基因特征.
一个表达S100A7/8/9和KRT6的新型HS特异性角质细胞群触发了炎症级联.
S100A8在调节HS角质细胞中的染色质可访问性方面发挥作用.
全基因组的染色质重新连接和转录因子切换介导HS转录特征.
在HS基底细胞中确定了临床相关的炎症增强剂及其相关的转录因子.
准S100A增强剂或IRF3降低HS相关的炎症调节剂.

结论:

皮肤上原始细胞的可塑性是HS病变的关键特征.
表观遗传失调,包括染色质重塑和增强剂活性,显著导致HS.
针对特定的S100A增强剂或IRF3是HS的有希望的治疗策略.

关键词:

在 IRF3 中,IRF3 是 IRF3 的一个类型.在S100A家族基因.表观遗传调节表观遗传调节性炎是一种补充性性炎.皮肤上皮细胞的皮肤上皮细胞.

更多相关视频

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Published on: February 27, 2020

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

相关概念视频

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Cadherins in Tissue Organization

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...