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多种CRISPR等离子体的扩大传播和分布模式由添加基因

Brook Pyhtila¹, Seth Kasowitz¹, Rachel Leeson¹

¹Addgene, Watertown, Massachusetts, USA.

The CRISPR journal

|November 27, 2023

概括

克里斯普尔等离子体的分布在全球范围内激增,突出了对各种基因组编辑工具的需求. 这反映了持续的创新和在研究,医学和农业的广泛采用.

科学领域:

遗传学和基因组学在
生物技术是生物技术.
分子生物学分子生物学

背景情况:

基于CRISPR的技术已经显著提升了基因组编辑能力.
目前,Addgene已经从1000多个实验室向全球5000多个组织分发了超过15,000个CRISPR等离子体.
对CRISPR等离子体的需求继续增加,这表明该领域正在迅速发展.

研究的目的:

分析过去十年CRISPR等离子体分布模式.
确定CRISPR效应器类型及其应用中的趋势.
了解CRISPR技术的全球采用和多样化.

主要方法:

分析等离子体沉积物,并向Addgene.com请求数据.
将CRISPR等离子体分类为核酶,调节器和化学效应器.
检查按地区和应用领域的请求趋势.

主要成果:

对于核酶 (Cas9,Cas12),调节器和仿真CRISPR效应器,存在强大的需求.
等离子体请求显示新沉积的结构的比例稳定,这表明持续的创新.
哺乳动物基因组操纵等离子体的需求最多,其次是对植物和微生物应用的需求不断增加.
全球分布覆盖大约100个国家,农业地区的需求日益增加.

结论:

在全球范围内,CRISPR技术的采用范围广泛且多样化.
新型CRISPR工具的持续开发支持人类健康和农业的多样化应用.
对植物和微生物CRISPR工具的日益增长的需求凸显了区域需求和应用.

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