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从暴露于缺氧的带血CD34+细胞中选和识别红细胞生产的参考基因

Jun Xiao¹, Zhicai Li², Xiaowei Li¹

¹Department of Blood Transfusion, Air Force Medical Center, Air Force Medical University, Beijing, China.

DNA and cell biology

|November 27, 2023

概括

确定稳定的参考基因对于带血CD34+细胞中精确的基因表达分析至关重要. 与GAPDH和HMBS不同的是,TBP和EIF4G2在不同氧气条件下表达最稳定.

科学领域:

血液形成和干细胞研究.
分子生物学和基因表达分析分析.

背景情况:

带血 (CB) CD34+细胞是人工血液形成的关键,但差异化机制尚不清楚.
准确的基因表达分析对于理解分化至关重要,它依赖于可靠的基因参考来进行定量实时PCR.

研究的目的:

在不同氧度下识别CB CD34+细胞的定量实时PCR的稳定基因.
在这种情况下,评估新型和传统参考基因对于正常化的适用性.

主要方法:

在CB CD34+细胞中选18个候选基因 (10个新基因,8个传统基因).
使用geNorm,NormFinder和RefFinder算法对基因转录稳定性的评估.
在不同的氧度条件下进行分析,以模拟差异化刺激.

主要成果:

确定TBP (传统) 和EIF4G2 (新型) 是最稳定的参考基因.
发现GAPDH和HMBS是最不稳定的,不适合正常化.
候选基因在不同氧气水平下表达稳定性在候选基因之间有显著差异.

结论:

建议TBP和EIF4G2作为可靠的基因参考基因,用于在不同氧气条件下分化CB CD34+细胞中的基因表达正常化.
这些发现为CB CD34+细胞分化的精确分子研究提供了基础.
避免在这个特定的实验环境中使用GAPDH和HMBS作为参考基因.

关键词:

红色素质的人类形成.基因表达的基因表达方式造血干细胞是造血干细胞的组成部分.缺氧缺氧是指缺氧的情况.基因基因基因基因基因基因基因基因基因基因

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Erythropoiesis

Erythropoiesis

Red blood cells (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia,...