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在COAD中Reg3A的瘤抑制作用是由裸体小鼠的T细胞激活介导的

Luting Yu¹, Yihan Zhou², Shaozheng Sun³

¹School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, China; School of Life Science & Technology, China Pharmaceutical University, Nanjing, China.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

|November 27, 2023

概括

再生家族蛋白3A (Reg3A) 通过激活T细胞,在结直肠癌 (CRC) 中表现出瘤抑制作用. 高Reg3A表达增强了抗瘤免疫力,改善了CRC患者的预后.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

再生家族蛋白3A (Reg3A) 与瘤发生和癌症进展有关.
临床数据表明,Reg3A在结直肠癌 (CRC) 中具有瘤抑制作用,尽管它在各种组织中表达很高.
在CRC中Reg3A的确切功能和机制尚不清楚.

研究的目的:

调查Reg3A表达,CRC发育和免疫细胞变化之间的关联.
为了阐明Reg3A在结肠腺癌 (COAD) 中的抗瘤机制.

主要方法:

使用TCGA,GEPIA,PrognoScan,TIMER和TISIDB数据库进行生物信息分析.
为异种移植小鼠模型构建一个Reg3A过度表达的LoVo细胞系 (LoVo-Reg3A).
通过RNA-seq和ex vivo共同培养实验评估Reg3A对瘤生长,亡,免疫细胞透和细胞因子产生的影响.

主要成果:

Reg3A上调与CRC相关,免疫细胞透率增加.
在异种移植模型中,Reg3A过度表达抑制了瘤增殖,并促进了亡.
Reg3A增强了T细胞的激活和招募,增加了CD4+,CD8+和CD3ε+T细胞,以及TNF-α,IFN-γ和IL-17的血清和细胞水平升高.

结论:

高Reg3A表达在COAD中具有细胞毒性瘤抑制作用.
Reg3A激活和招募T细胞,从而增强抗瘤免疫力.

关键词:

它具有抗瘤作用.尾巴尾巴尾巴尾巴尾巴尾巴结肠直肠癌是一种癌症.裸露的小鼠模型在RNA-seqqq.再生蛋白质可以再生蛋白质.在T细胞,T细胞.

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