针对2型糖尿病的无标签定量蛋白质组学分析,使用数据独立采集质谱法 (DIA-MS) 发现早期诊断标记
Refat M Nimer1, Mahmoud A Alfaqih2,3, Eman R Shehabat4
1Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, 22110, Jordan. rmnimer@just.edu.jo.
Scientific reports
|November 27, 2023
概括
这项研究使用质谱学确定了用于早期2型糖尿病 (T2DM) 诊断的新型蛋白质生物标志物. 在T2DM患者中,S100A6的水平增加,表明其作为诊断标记物的潜力.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 生物标志物发现发现
- 代谢学 代谢学 代谢学
背景情况:
- 目前2型糖尿病 (T2DM) 的诊断标志物不足以用于早期检测和预防并发症.
- 数据独立采集质谱 (DIA-MS) 为临床诊断中精确量化提供了可靠的方法.
研究的目的:
- 通过使用DIA-MS. 来调查最近被诊断为T2DM患者血清中的蛋白质差异表达.
- 识别和验证用于早期T2DM诊断的新型蛋白质生物标志物.
主要方法:
- 使用DIA-MS (发现队列) 对健康对照和最近诊断的T2DM患者的血清样本进行比较蛋白质组分析.
- 在单独的队列中使用酶相关免疫检测 (ELISA) 验证候选蛋白质生物标志物 (S100A6).
- 接收器操作特征 (ROC) 曲线分析用于生物标志物评估.
主要成果:
- 确定了1074种蛋白质,其中90种在T2DM和对照组之间显著失调.
- 发现了32种与T2DM发育和进展相关的新型蛋白质.
- 在T2DM患者中通过ELISA验证了S100结合蛋白A6 (S100A6) 表达的增加,证实了其作为生物标志物的潜力.
结论:
- 在T2DM中识别差异性蛋白质表达时,DIA-MS是有效的.
- S100A6是早期T2DM检测的一个有前途的新生物标志物.
- 需要进一步的大规模研究来确认在T2DM患者中确定候选标记物的有用性.
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