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野生室内小鼠的替代拼接和环境适应

David N Manahan¹, Michael W Nachman²

¹Department of Integrative Biology and Museum of Vertebrate Zoology, University of California, Berkeley, CA, 94720, USA. david_manahan@berkeley.edu.

|November 27, 2023

概括

替代拼接是一种基因调节形式,有助于室内小鼠的适应. 这项研究确定了与环境适应相关的特定的替代拼接转录,与基因表达的变化不同.

科学领域:

进化遗传学的进化遗传学
适应的分子机制.
基因调节基因调节

背景情况:

适应性进化是由遗传和分子变化驱动的.
基因调节,特别是mRNA丰富度,是已知的适应贡献者.
在适应过程中,像替代拼接一样,转录后处理的作用是不太了解的.

研究的目的:

通过使用替代拼接在室内小鼠中识别局部适应的候选基因.
研究替代拼接在沿着度梯度的环境适应中的作用.
为了区分替代拼接与mRNA丰度变化的适应性作用.

主要方法:

来自野生室内小鼠 (Mus musculus domesticus) 的外体序列和RNA-Seq数据的分析.
识别具有种群特异性和临床变异的替代拼接转录.
协会研究以检测cis作用拼接定量特征位点 (cis-sQTL).
叠加分析与从基因组扫描中选择的目标.

主要成果:

识别了替代拼接的成绩单,显示了横跨度横断线的临床变化.
发现cis-sQTL与已知的选择标重叠.
发现许多候选基因与身体大小有关,这是临床上不同的特征.
没有观察到与之前通过mRNA丰度变化识别的基因的重叠.

结论:

替代拼接是一种独特的分子机制,有助于室内小鼠的环境适应.
这些发现强调了转录后调节在进化适应中的重要性.
替代拼接和mRNA丰度的变化可能会作为适应性进化的单独途径.

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