抗原呈现细胞的活化动态体内针对Mycobacterium bovis BCG在不同的免疫路径
Zhengzhong Xu1,2, Xin Li2, Aihong Xia1
1Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, Jiangsu, 225009, China.
细菌卡尔梅特-盖林 (BCG) 感染的途径影响T细胞反应和抗原呈现细胞 (APC) 激活. 与皮下BCG感染相比,静脉BCG感染导致脏APC激活速度更快.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 疫苗学 疫苗学 疫苗学
背景情况:
- 结核病 (TB) 控制依赖于T细胞和抗原呈现细胞 (APC) 的合作.
- 在Bacillus Calmette-Guérin (BCG) 感染期间,细胞免疫反应部的早期APC激活动态尚不清楚.
研究的目的:
- 研究BCG注射途径对APC激活和T细胞反应的影响.
- 为了比较mycobacterial吸收,细胞因子的生产,抗原呈现,和细胞表型的APCs.
主要方法:
- C57BL/6小鼠被输入静脉 (IV) 感染. 或通过BCG进行皮下注射 (s.c.).
- 排序了脏和 inguinal 淋巴结 (LN) 树突细胞 (DC) 和巨细胞 (MΦs).
- 分析了菌根菌的吸收,细胞因子的产生,抗原呈现和细胞表型.
主要成果:
- 在BCG感染后6天开始的Ag85A特异性T细胞反应.
- 皮下BCG诱导了 inguinal LNs.中的Th17反应.
- BCG增强了 inguinal LN MΦ 的激活,但 DCs 显示了 mycobacterial 抗原的优越呈现.
结论:
- BCG免疫接种的路线决定了T细胞免疫反应的主要部位.
- 与皮下途径相比,静脉BCG感染导致在脏中的APC激活速度更快.
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