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通过与FUS相互作用,CircHIPK3通过调解SIRT1/PGC-1α/MFN2通路缓解血管化

Siyi Feng¹, Youfei Qi², Zhanxiang Xiao²

¹Department of Ultrasound Medicine, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, 570311, Hainan Province, China.

BMC cardiovascular disorders

|November 27, 2023

概括

循环HIPK3 (circHIPK3) 水平在血管化中降低. 恢复circHIPK3通过稳定SIRT1和激活PGC-1α/MFN2通路来抑制沉积,提供了一个新的治疗点.

科学领域:

分子生物学分子生物学
心血管研究研究心血管研究
基因规则基因规则

背景情况:

循环RNAs (circRNAs) 在人类疾病中发挥作用.
CircHIPK3与血管化有关,但其机制尚不清楚.
这项研究研究了circHIPK3在血管化中的调节作用.

研究的目的:

阐明circHIPK3在血管化中的下游调节机制.
确定circHIPK3如何影响血管光滑肌细胞 (VSMC) 化.
为了确定血管化潜在的治疗点.

主要方法:

定量实时PCR (qRT-PCR) 来评估circHIPK3的表达.
RNA免疫沉 (RIP) 和拉下测试以验证结合相互作用.
性酸酶 (ALP) 活性和阿利沙林红色染色以测量沉积.
西方斑点用于分析VSMC中的蛋白质表达.

主要成果:

在动脉样硬化血清和VSMC化模型中,circHIPK3的表达减少.
在VSMC中,circHIPK3的过度表达抑制了ALP活性和沉积.
通过招募FUS,CircHIPK3稳定了SIRT1mRNA.
CircHIPK3促进了MFN2的表达,通过SIRT1/PGC-1α通路缓解了VSMC化.

结论:

该circHIPK3/FUS/SIRT1/PGC-1α/MFN2通路积极调节和缓解VSMC化.
这一途径代表了血管化的新型调节轴.
CircHIPK3有可能成为血管化的治疗标.

关键词:

化是一种化过程.电路IPK3K3 电路IPK3K3 这就是FUS FUS.通过SIRT1/PGC-1α信号传输.

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