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Updated: Jul 9, 2025

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.
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A Simple Chelex Protocol for DNA Extraction from Anopheles spp.

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从抛化脏样本中提取DNA的标准化:人类疟疾分子诊断

Raimunda Sandra Pacheco Souza1, Monique F Dos Reis2, Luiz Carlos de Lima Ferreira3,4

  • 1Leonidas & Maria Deane Institute (ILMD), Fiocruz, Manaus, Amazonas, 69057-070, Brazil.

Malaria journal
|November 28, 2023
PubMed
概括

研究人员标准化了从脏组织中提取DNA,用于诊断Plasmodium vivax疟疾. 这种方法可以更好地检测脏疟疾,这是巴西具有挑战性的诊断.

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DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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