硫胺 硫胺的形成来自基和氧化的反应
Alberto Negrellos1, Allison M Rice1, Patricia C Dos Santos1
1Department of Chemistry, Wake Forest University, Winston-Salem, North Carolina 27107, United States.
ACS chemical biology
|November 28, 2023
概括
细菌中的一个关键醇 - - 乙醇 (BSH) - - 与氧化 (HNO) 发生独特的反应,形成硫胺,与谷氨不同. 这种反应对于暴露于HNO的细菌生存和毒性至关重要.
科学领域:
- 微生物学 微生物学
- 生物化学 生化学
- 化学生物学 化学生物学
背景情况:
- 乙乙醇 (BSH) 是许多格拉姆阳性细菌中主要的低分子量醇,对于金属结合,解毒和毒性至关重要.
- 由于天然来源有限和合成复杂,BSH与氧化 (HNO) 等反应性物种的活性在很大程度上尚未被探索.
研究的目的:
- 研究乙醇 (BSH) 和氧化 (HNO) 之间的化学反应.
- 阐明细菌中BSH-HNO相互作用的生物学意义,特别是细菌细菌.
主要方法:
- 化学合成和BSH的制备.
- 从安吉利盐中产生的氧化 (HNO) 对BSH的暴露.
- 使用染色学和质谱学分析反应产品.
- 在HNO暴露下使用* Bacillus subtilis*菌株进行体内实验.
主要成果:
- BSH与HNO反应,只形成硫胺产物,包括一种新型的循环硫胺,与谷氨的反应产物不同.
- BSH的酸部分通过N-hydroxysulfenamide中间体参与硫胺的形成.
- 在体内, * Bacillus subtilis * 的生长受到 HNO 的影响,缺乏 BSH 的菌株表现出过敏.
- 硫醇分析显示,降低了降低的BSH水平,而没有增加二硫化物形成,支持硫胺衍生物的形成.
结论:
- 巴西利醇与氧化具有独特的化学反应,形成硫胺,这是与谷氨酸不同的途径.
- 在BSH中酸组对于这种独特的硫胺形成机制至关重要.
- BSH-HNO相互作用具有生物学相关性,影响细菌生长和存活,突出了BSH在细菌防御活性物种中的作用.
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