从地中分离出来的NDM-1阳性Citrobacter freundii菌株的表征
Xiaoyang Ju1, Yanyan Zhu1, Panfeng Xiong2
1Department of Clinical Laboratory, The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, China.
Journal of global antimicrobial resistance
|November 28, 2023
概括
虫可以携带NDM-1碳烯耐药基因,正如从它们的肠道中分离出耐药细菌所示. 这凸显了对抗菌素耐药性的环境监测的必要性.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 一个健康,一个健康.
背景情况:
- 虫是重要的土壤生物,参与营养循环和污染物转化.
- 抗菌素耐药性 (AMR) 是一个日益严重的全球健康问题,需要识别新的储存和传播途径.
研究的目的:
- 描述NDM-1产生的 *C. freundii* 从医院附近的地中分离出来.
- 研究虫作为耐卡巴胺基因的载体的潜在作用.
主要方法:
- 使用MALDI-TOF MS.分离和识别来自肠中的细菌.
- 通过CARBA-5测试验证NDM酶的存在.
- 全基因组测序,抗菌素敏感性测试和结合实验.
主要成果:
- 鉴定出一种多药耐药的 *C. freundii* 隔离物,该隔离物耐受于碳烯.
- blaNDM-1基因被成功转移到大肠杆菌中,并被发现在IncFIIY型等离子体上.
- 遗传学分析表明,土虫分离物与来自同一地区的人类分离物之间存在密切的关系.
结论:
- 这是从虫肠道中分离出来的NDM-1产生细菌的第一份报告.
- 虫可能会成为抗卡巴胺基因的潜在储存库.
- 加强对AMR的环境监测至关重要.
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