基因鉴定和半合成的抗炎性oleanane类型的三基因wilforlide A A的基因鉴定和半合成
Yuan Liu1,2, Jiawei Zhou1,3, Panting Liu1
1School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, China.
The New phytologist
|November 28, 2023
概括
研究人员确定了合成wilforlide A的关键基因,这是一种具有抗炎性质的化合物. 他们设计了酵母来生产wilforlide A,提供了一个独立于植物的生产方法.
科学领域:
- 自然产品生物合成 自然产品生物合成
- 代谢工程是代谢工程.
- 分子生物学分子生物学
背景情况:
- 威尔福利德A是Tripterygium wilfordii Hook F的关键活性化合物,具有显著的抗炎和免疫抑制作用.
- 威尔福利德A的生物合成途径,尽管结构简单,但以前没有被阐明.
- 了解威尔福利德A生物合成对于开发替代生产战略至关重要.
研究的目的:
- 鉴定和描述涉及威尔福利德A.生物合成的基因.
- 建立一种代谢工程方法,用于在异质宿主中产生wilforlide A.
- 通过途径复制使wilforlide A的半合成成为可能.
主要方法:
- 基因特异性表达分析和基因组挖掘以确定候选基因.
- 鉴定基因的体外和体外功能特征.
- 使用模块化两步 (M2S) 和CRISPR-Cas9技术构建工程酵母.
主要成果:
- 在wilforlide A生物合成中催化C-22氧化C-450 TwCYP82AS1细胞染色体的鉴定.
- 发现TwCYP712K1到K3酶氧化C-29氧化在烯和烯类型三烯中.
- 在工程酵母中成功复制生物合成途径,从而产生abrusgenic酸.
结论:
- 这项研究阐明了wilforlide A生物合成中的关键酶性步骤.
- 工程酵母成功生产了abrusgenic acid,这是wilforlide A半合成的前体.
- 这项工作提供了一个新的代谢工程策略,用于生产wilforlide A,独立于植物提取.
更多相关视频
07:59A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
9.8K
11:44Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
Published on: January 19, 2022
2.5K
相关概念视频
02:27DNA Base Pairing
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
01:11Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase
Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
01:29Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
01:28Inhibitors of Bacterial DNA Synthesis
Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These antibiotics are selectively...