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基于calixarene的冷保护剂用于抑制冰的再结晶和细胞冷保存

Bing Hu¹, Juan-Juan Li², Yan-Bin Ren¹

¹College of Chemistry and Materials Science, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Ministry of Education), State Key Laboratory of New Pharmaceutical Preparations and Excipients, Key Laboratory of Chemical Biology of Hebei Province, Hebei University, Baoding, 071002, China. kerangwang@hbu.edu.cn.

Journal of materials chemistry. B

|November 28, 2023

概括

为细胞冷保存开发了新的基于卡利沙的冷保护剂 (CPAs). SBAC4A和SC4A表现出优异的冷保护作用,增强了各种细胞类型的细胞活力.

科学领域:

超分子化学超分子化学
生物材料科学生物材料科学
细胞生物学细胞生物学

背景情况:

细胞的冷保存对于各种生物和医学应用至关重要.
开发有效的冷保护剂 (CPA) 对于提高细胞存活率至关重要.
基于calixarene的化合物为新型CPA设计提供了一个有前途的支架.

研究的目的:

综合和描述五种新的基于卡利沙林的CPAs.
评估这些新型CPAs的冷保护功效.
建立一个超分子原理来设计先进的CPA.

主要方法:

合成了五种基于卡力沙林的CPA:SC4A,S-S-C4A,S-SO2-C4A,SBAC4A和CAC4A.
评估IRI活动和DIS属性.
对MCF-7,hucMSCs和A549细胞的冷保护作用的评估.

主要成果:

与其他合成CPA相比,SBAC4A和SC4A表现出优越的冷保护作用.
SBAC4A显著提高了细胞活力:MCF-7的16.16 ± 1.78%,hucMSC的12.60 ± 1.15%,A549细胞的14.90 ± 1.66%.
该研究确定了影响CPA表现的关键因素,包括结,宏分子拥挤和3D结构.

结论:

基于卡利沙林的CPA,特别是SBAC4A和SC4A,显示出细胞冷保存的巨大潜力.
这些发现为开发下一代CPA提供了一个超分子设计策略.
了解分子相互作用和结构特征的相互作用是优化冷保护活性的关键.

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