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在双光子电压成像上的光学约束

F Phil Brooks¹, Hunter C Davis¹, J David Wong-Campos¹

¹Department of Chemistry and Chemical Biology, Harvard University.

bioRxiv : the preprint server for biology

|November 28, 2023

概括

两光子 (2P) 电压成像需要更大的功率,并且可以比一光子 (1P) 成像更少的细胞成像. 在深度中实现许多神经元的高SNR2P成像需要改进的基因编码电压指示器 (GEVIs) 或新的成像技术.

科学领域:

神经科学是一个神经科学.
光学成像技术的成像
生物物理学的生物物理.

背景情况:

在体内神经电路研究中,基因编码的电压指示器 (GEVIs) 是至关重要的.
一光子 (1P) 和两光子 (2P) 电压成像的比较性能需要详细的描述.

研究的目的:

为了比较1P和2P电压成像的光学和生物物理限制.
在1P和2P激发下评估常见GEVI的成像特性.

主要方法:

在1P和2P照明下测量了GEVI的亮度和电压灵敏度.
在小鼠大脑中,量化光会随着深度的增加而减少.
开发了一个模型来预测可测量的细胞,基于记者属性,成像参数和SNR.

主要成果:

2P激发需要每单元约10^4倍的照明功率,而不是相似的光子速率的1P.
在特定条件下 (JEDI-2P,SNR 10,1kHz,200mW,深度>300μm),2P成像只从12个细胞同时捕获数据.

结论:

由于严格的光子要求,现有的2P GEVIs面临着射击噪声和光损伤之间的关键权衡.
在300微米以上的深度对数百个具有高SNR的神经元进行成像,需要大幅改善GEVI或新的成像方式.

关键词:

听到了枪声的声音.两个光子的两个光子.电压成像技术电压成像技术

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