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相关概念视频

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

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A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
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Photoelectric Effect02:26

Photoelectric Effect

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When light of a particular wavelength strikes a metal surface, electrons are emitted. This is called the photoelectric effect. The minimum frequency of light that can cause such emission of electrons is called the threshold frequency, which is specific to the metal. Light with a frequency lower than the threshold frequency, even if it is of high intensity, cannot initiate the emission of electrons. However, when the frequency is higher than the threshold value, the number of electrons ejected...
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相关实验视频

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Direct Imaging of Laser-driven Ultrafast Molecular Rotation
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Direct Imaging of Laser-driven Ultrafast Molecular Rotation

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在双光子电压成像上的光学约束.

F Phil Brooks1, Hunter C Davis1, J David Wong-Campos1

  • 1Department of Chemistry and Chemical Biology, Harvard University.

bioRxiv : the preprint server for biology
|November 28, 2023
PubMed
概括

两光子 (2P) 电压成像需要更大的功率,并且可以比一光子 (1P) 成像更少的细胞成像. 在深度中实现许多神经元的高SNR2P成像需要改进的基因编码电压指示器 (GEVIs) 或新的成像技术.

科学领域:

  • 神经科学是一个神经科学.
  • 光学成像技术的成像
  • 生物物理学的生物物理.

背景情况:

  • 在体内神经电路研究中,基因编码的电压指示器 (GEVIs) 是至关重要的.
  • 一光子 (1P) 和两光子 (2P) 电压成像的比较性能需要详细的描述.

研究的目的:

  • 为了比较1P和2P电压成像的光学和生物物理限制.
  • 在1P和2P激发下评估常见GEVI的成像特性.

主要方法:

  • 在1P和2P照明下测量了GEVI的亮度和电压灵敏度.
  • 在小鼠大脑中,量化光会随着深度的增加而减少.
  • 开发了一个模型来预测可测量的细胞,基于记者属性,成像参数和SNR.

主要成果:

  • 2P激发需要每单元约10^4倍的照明功率,而不是相似的光子速率的1P.
  • 在特定条件下 (JEDI-2P,SNR 10,1kHz,200mW,深度>300μm),2P成像只从12个细胞同时捕获数据.

结论:

  • 由于严格的光子要求,现有的2P GEVIs面临着射击噪声和光损伤之间的关键权衡.
关键词:
听到了枪声的声音.两个光子的两个光子.电压成像技术 电压成像技术

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  • 在300微米以上的深度对数百个具有高SNR的神经元进行成像,需要大幅改善GEVI或新的成像方式.