跨-eQTL热点通过调节细胞状态来塑造复杂的特征
Kaushik Renganaath1, Frank W Albert1
1Department of Genetics, Cell Biology, & Development, University of Minnesota, Minneapolis, MN 55455, USA.
bioRxiv : the preprint server for biology
|November 28, 2023
概括
影响基因表达的遗传变异显著影响复杂的特征. 我们的研究表明,跨作用调节热点,而不是局部变异,是酵母生长中这些连接的关键驱动因素.
科学领域:
- 遗传学 遗传学 是一个
- 系统生物学 系统生物学
- 分子生物学分子生物学
背景情况:
- 调节性遗传变异影响基因表达,将DNA变异与复杂的特征联系起来.
- 基因表达和复杂特征之间的确切因果关系尚未得到充分理解.
- 对于从医学到进化生物学等各个领域来说,理解这些联系至关重要.
研究的目的:
- 研究酵母菌中基因表达和复杂生长特征之间的关系.
- 确定基因表达变异的基因架构及其对复杂特征的影响.
- 阐明局部与跨作用调节变异在塑造复杂特征中的作用.
主要方法:
- 在酵母交叉 (Saccharomyces cerevisiae) 中整合了转录基因数据与46个遗传复杂的生长特征.
- 分析基因表达和生长特征之间的遗传相关性.
- 区分地方监管变化的贡献与多个独立的跨作用位置的贡献.
主要成果:
- 在基因表达和酵母菌生长之间发现了成千上万的遗传相关性.
- 局部调节变异是较小的贡献者;跨作用的调节位置是遗传相关性的主要来源.
- 跨作用调节热点显著影响了基因生长变异和基因表达-生长相关性,影响了许多基因.
结论:
- 跨作用调节热点是通过调节细胞状态来决定复杂特征的主要决定因素.
- 了解这些热点可以了解遗传变异如何转化为表型多样性.
- 这项研究强调了非局部监管要素在塑造生物复杂性的重要性.
相关概念视频
General Transcription Factors
5.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.3K
Overview of Transposition and Recombination
15.5K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.5K
Transcription Factors
75.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
75.9K
Position-effect Variegation
6.3K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.3K
Chromatin Position Affects Gene Expression
23.3K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.3K
Gene-Environment Interactions
328
Gene expression is a dynamic process that is significantly influenced by environmental factors. This interaction underlies the complex nature of biological development and the phenotypic differences observed among individuals, even among those with identical genetic makeups. Factors such as radiation, temperature, behavior, nutrition, and stress play pivotal roles in determining how genes are expressed. The concept of the reaction range is central to understanding this interaction. It posits...
328


