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相关概念视频

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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相关实验视频

Updated: Jun 14, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

COL:一个管道用于识别假定功能性的回合拼接.

Zheng Li, Bandhan Sarker, Fengyu Zhao

    bioRxiv : the preprint server for biology
    |November 28, 2023
    PubMed
    概括
    此摘要是机器生成的。

    识别功能性循环RNA (circRNAs) 是一个挑战. 一个新的计算管道,COL,有效地预测功能性circRNAs使用保存的拼接动机和高的反拼接率从单个样本.

    更多相关视频

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
    08:35

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

    Published on: June 24, 2021

    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
    08:53

    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

    Published on: September 15, 2021

    相关实验视频

    Last Updated: Jun 14, 2026

    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
    09:58

    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

    Published on: December 9, 2016

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
    08:35

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

    Published on: June 24, 2021

    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
    08:53

    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

    Published on: September 15, 2021

    科学领域:

    • 分子生物学分子生物学
    • 生物信息学是一种生物信息学.
    • 基因组学就是基因组学.

    背景情况:

    • 循环RNAs (circRNAs) 是通过反向拼接形成的非编码RNA.
    • 虽然大多数circRNAs是非功能性的,但一个子集具有生物学作用.
    • 将功能性circRNA与非功能性circRNA区分开来至关重要,但由于当前实验和计算方法的局限性,这是很困难的.

    研究的目的:

    • 开发一种新的计算方法来识别假定功能性circRNAs.
    • 解决低通量实验技术和不可靠的计算方法的局限性.

    主要方法:

    • 这项研究假设功能背接事件的发生率高于预期,具有保存的拼接动机,并显示异常高的背接水平.
    • 这些特征在人类,和小鼠的保存后接合事件中得到证实.
    • 设计了一个名为 COL 的计算管道,将这三个特征集成在一起,以预测功能背接.

    主要成果:

    • COL可以使用单个生物样本来预测功能背接,而不是需要多个样本的方法.
    • 与仅依赖背接水平的方法相比,COL的虚假阳性率较低.
    • 管道成功识别了可谓的功能背接事件.

    结论:

    • COL是一种高效和通用的计算工具,用于快速识别潜在的功能性circRNAs.
    • 已识别的circRNAs适合进行实验验证.
    • COL是公开的,促进了该领域的进一步研究.