相关实验视频

Updated: Jul 9, 2025

Investigating Long-Distance Transport of Perfluoroalkyl Acids in Wheat via a Split-Root Exposure Technique

Investigating Long-Distance Transport of Perfluoroalkyl Acids in Wheat via a Split-Root Exposure Technique

Published on: September 28, 2022

从五种结构类别中对五种结构类别的Per-和多基基物质的血清白蛋白结合的定量跨物种比较

Hannah M Starnes¹, Thomas W Jackson^1,2, Kylie D Rock^1,3

¹Department of Biological Sciences, North Carolina State University, 127 David Clark Labs Campus Box 7617, Raleigh, NC 27607, USA.

bioRxiv : the preprint server for biology

|November 28, 2023

概括

血清白蛋白与各种各样的per-和多醇基物质 (PFAS) 结合,在不同物种中观察到显著差异. 这项研究澄清了PFAS的结合 afinities,改善了毒性预测.

科学领域:

环境化学环境化学
毒理学毒理学毒理学
生物化学生物化学

背景情况:

和多基基物质 (PFAS) 是广泛,持久,生物积累和有毒的化学物质.
了解PFAS与白蛋白等血清蛋白结合,对于评估毒性至关重要.
现有的关于PFAS与白蛋白结合的实验数据仅限于少数化合物.

研究的目的:

为了比较分析人类,牛,猪和老鼠血清白蛋白对八种结构信息的PFAS原体的结合亲和力.
为了研究PFAS血清白蛋白结合的物种间差异.
减少PFAS预测毒性评估中的不确定性.

主要方法:

使用了高通量体外差分扫描度测试 (DSF) 试验.
评估了四种物种 (人类,牛,猪,老鼠) 血清白蛋白的相对结合亲缘关系.
研究了五个化学类别的八种结构多样化的PFAS类别.

主要成果:

大多数与人血清白蛋白结合的PFAS也与牛,猪和大鼠血清白蛋白结合,但6:2 FTOH除外.
充电的功能头组对于白蛋白结合至关重要,这是6:2 FTOH缺乏结合的证据.
在结合亲和性方面观察到显著的物种间差异;例如,猪和老鼠蛋白对某些PFAS酸的亲和力比人类蛋白更高.

结论:

对跨物种的PFAS-albumin结合的比较分析为毒性评估提供了有价值的数据.
结合性亲缘关系的跨物种差异凸显了在风险评估中需要对特定物种进行考虑的需要.
该研究通过减少物种间不确定性,提高了PFAS预测毒性模型的准确性.

关键词:

在PFAS中,我们可以使用PFAS.结合性亲和力是一种结合性亲和力.不同扫描光仪的差异扫描光仪.在体外 (in vitro) 的情况下.种类抽取取值种类抽取值

更多相关视频

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

Published on: October 16, 2015

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Published on: April 18, 2019

相关概念视频

Protein-Drug Binding: Determination Methods

Protein-Drug Binding: Determination Methods

Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...