突变效应的环境独立分布从微观表观中出现
Sarah Ardell1, Alena Martsul1, Milo S Johnson2,3
1Department of Ecology, Behavior and Evolution, University of California San Diego, La Jolla, CA 92093.
bioRxiv : the preprint server for biology
|November 28, 2023
概括
预测突变对特征的影响是具有挑战性的. 全球表现有帮助,但环境影响尚不清楚. 这项研究揭示了环境调整单个参数,使突变效应可预测跨条件.
科学领域:
- 进化遗传学的进化遗传学
- 定量遗传学 是一种定量遗传学.
- 酵母遗传学 酵母遗传学
背景情况:
- 由于基因型和环境相互作用,对表型的突变影响的预测是复杂的.
- 全球表现症,其中突变效应取决于遗传背景适应性,提供了一个潜在的预测框架.
- 环境变化的影响全球表观缩扩展在很大程度上仍未被探索.
研究的目的:
- 调查环境因素如何调节由全球表观病治下的突变效应的扩大.
- 为了确定全球表观是否提供了一个一致的框架来预测跨不同环境的突变影响.
- 了解在不同的环境条件下基因型-表型关系的可预测性.
主要方法:
- 在42个Saccharomyces cerevisiae菌株中对大约100个插入突变的适应性测试.
- 在六个不同的实验室环境中的实验进化.
- 在每个环境中分析突变效应分布和全球表现性参数的分析.
主要成果:
- 在六个测试环境中,全球表达性扩展在很大程度上是不变的.
- 环境调节主要通过单一的全球参数发生:信号切换突变的背景适应值.
- 突变效应的分布在不同的基因型和环境中表现出可预测性.
结论:
- 环境对突变结果的影响可以通过低维基基因型-表型映射来简化.
- 全球表征为预测突变效应提供了一个强大的框架,环境调整以关键的健康参数为中心.
- 了解这些原则可以提高对进化轨迹和表型变异的预测.
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