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在急性呼吸窘迫综合征的支气管膜洗中再生签名

Runzhen Zhao^1,2, Marco Hadisurya³, Harrison Ndetan⁴

¹Department of Surgery, Stritch School of Medicine, Loyola University Chicago Health Sciences Division, Maywood, IL 60153, USA.

bioRxiv : the preprint server for biology

|November 28, 2023

概括

研究人员在急性呼吸困扰综合征 (ARDS) 患者中发现了新型外体蛋白质,揭示了肺部修复的潜在再生. 这些发现为ARDS肺部再生机制提供了新的见解.

科学领域:

肺部医学肺部医学
再生医学是一种再生医学.
蛋白质组学是指蛋白质组学.

背景情况:

与败血症相关的严重急性呼吸困扰综合征 (ARDS) 会导致肺部恢复延迟和高死亡率.
了解ARDS肺部再生机制对于改善患者的治疗结果至关重要.
外基因组在细胞间通信中起着至关重要的作用,并参与组织修复过程.

研究的目的:

通过对来自ARDS患者的支气管膜洗 (BAL) 外体细胞中差异表达蛋白质的特征来识别潜在的再生.
调查外体蛋白在ARDS肺部再生和再膜化中的作用.
为了将外体蛋白质形状与ARDS严重程度和临床变量相关联.

主要方法:

从NIH支持的ALTA和SPIROMICS试验中的ARDS患者的BAL样本中净化了外体.
使用液态染色体质谱法 (LC-MS) 量化了外体蛋白和的丰度.
差异表达的外体蛋白质被分析为功能注释,细胞起源,信号通路和临床相关性.

主要成果:

在晚期ARDS患者中发现了更多的外体蛋白质.
免疫细胞和肺上皮干细胞是BAL外体的主要贡献者.
在ARDS患者中观察到丰富的功能,干细胞信号,生长因子和免疫.
差异表达蛋白与关键临床变量相关,并注意到与严重程度相关的网络差异.
膜2型上皮细胞被确定为外体细胞的捐赠者和接受者.

结论:

在ARDS洗样本中发现了与各种功能,信号通路和细胞起源相关的新型外体蛋白.
这些已识别的蛋白质可能代表了在受伤的肺部中重新膜化新的再生.
这项研究为开发基于外体的ARDS治疗策略提供了基础.

关键词:

急性呼吸困扰综合征是什么支气管气管洗支气管气管气管洗外基因组是外基因组的一个组成部分.蛋白质组学蛋白质组学复兴再生是一种再生方式.

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