酸影响所有细胞中的细胞增殖,细胞亡和氧化应激
Büşra Hilal1, Aslı Eldem2, Tuba Oz2
1Faculty of Medicine, Izmir Katip Celebi University, Izmir, Turkey.
Biological trace element research
|November 28, 2023
概括
酸 (BA) 显示出作为T细胞急性淋巴细胞白血病 (T-ALL) 的抗癌剂的潜力. 它抑制了癌细胞的增殖,并诱导了细胞灭绝,提供了一种不那么有毒的治疗选择.
科学领域:
- 在瘤学瘤学.
- 生物化学 生化学
- 分子生物学分子生物学
背景情况:
- T细胞急性淋巴细胞白血病 (T-ALL) 由于化疗耐药性和需要更少的毒性疗法而面临挑战.
- 元素,特别是酸 (BA),对癌细胞具有细胞毒性,需要进一步研究.
研究的目的:
- 为了研究酸 (BA) 对Jurkat细胞的细胞增殖,细胞亡和氧化应激的影响,T-ALL的模型.
- 评估BA作为T-ALL的抗癌治疗剂的潜力.
主要方法:
- 使用XTT试验评估细胞活力以确定IC50度.
- 通过阿克里丁色/乙化染色分析的亡以及使用RT-qPCR的亡调节剂 (Bcl-2,Bax,Caspase-3-8-9) 和miR-21的基因表达.
- 氧化应激标志物 (TOS,TAS,OSI) 被量化.
主要成果:
- 酸证明了对Jurkat细胞增殖的剂量依赖抑制,IC50为802.7μg/mL.
- 通过降低对抗亡的基因的调节和提高对亡的基因的调节,BA诱导了亡,同时显著减少了miR-21表达.
- 在Jurkat细胞中,BA治疗增加了总抗氧化剂状态 (TAS) 并降低了总氧化剂状态 (TOS).
结论:
- 酸对Jurkat细胞表现出细胞毒性作用,抑制增殖并诱导亡.
- BA调节了关键的亡相关基因和微RNA的表达,这表明它在编程细胞死亡中发挥了作用.
- 这些发现支持酸作为T-ALL的潜在抗癌药物,通过抗增殖和促细胞亡机制作用,对细胞氧化还原平衡产生影响.
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