Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.3K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.3K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Nano-electron volt Fourier-limited transition of a single surface-adsorbed molecule.

Science (New York, N.Y.)·2026
Same author

Red blood cell-derived extracellular vesicles as biomaterials: the opportunity of freezing-induced accelerated aging.

Biomaterials science·2025
Same author

Cryogenic light microscopy of vitrified samples with angstrom precision.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Bottom-up Investigation of Spatiotemporal Glycocalyx Dynamics with Interferometric Scattering Microscopy.

Journal of the American Chemical Society·2025
Same author

Cryo-light microscopy with angstrom precision deciphers structural conformations of PIEZO1 in its native state.

Science advances·2025
Same author

Hybridization of molecules via a common photonic mode.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Beyond the scaffold: extracellular matrix uptake in breast cancer.

Biochemical Society transactions·2026
Same journal

TDP-43 proteinopathy as a biomarker and therapeutic target in amyotrophic lateral sclerosis.

Biochemical Society transactions·2026
Same journal

Advancing the monitoring of organelle contact sites in vitro and in vivo.

Biochemical Society transactions·2026
Same journal

Mechanisms influencing transient cytoplasmic protein targeting to intracellular lipid droplets.

Biochemical Society transactions·2026
Same journal

Replication associated nuclear DNA mismatch repair across kingdoms.

Biochemical Society transactions·2026
Same journal

Phosphatases of regenerating liver downregulate PTEN to promote tumorigenesis.

Biochemical Society transactions·2026
查看所有相关文章

相关实验视频

Updated: Jul 9, 2025

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
09:37

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Published on: August 15, 2014

43.7K

使用冷光显微镜对蛋白质结构的洞察力.

Hisham Mazal1,2, Franz-Ferdinand Wieser1,2,3, Vahid Sandoghdar1,2,3

  • 1Max Planck Institute for the Science of Light, 91058 Erlangen, Germany.

Biochemical Society transactions
|November 28, 2023
PubMed
概括
此摘要是机器生成的。

低温光显微镜 (Cryo-LM) 通过减少样品运动和光漂白来克服生物成像中的分辨率限制. 这种技术使得蛋白质和蛋白质复合体的高精度结构分析成为可能.

科学领域:

  • 生物物理学的生物物理.
  • 显微镜的使用方法
  • 结构生物学 结构生物学
关键词:
相关成像成像是相关的成像.低温电磁波冷却器 (Cryo-EM) 是一个非常好的方法.制冷式超分辨率超级分辨率光是一种光.蛋白质的结构和组合.

更多相关视频

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
09:16

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Published on: February 7, 2022

6.4K
Optimizing Sample Preparation for Cryogenic Electron Microscopy
06:32

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Published on: April 11, 2025

434

相关实验视频

Last Updated: Jul 9, 2025

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
09:37

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Published on: August 15, 2014

43.7K
Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
09:16

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Published on: February 7, 2022

6.4K
Optimizing Sample Preparation for Cryogenic Electron Microscopy
06:32

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Published on: April 11, 2025

434

背景情况:

  • 像超分辨率这样的先进光显微镜方法改善了生物样本成像.
  • 样本运动和光漂白限制了传统显微镜所能达到的分辨率.
  • 通常需要化学固定,这限制了动态研究.

结论:

  • Cryo-LM代表了光显微镜的重大进步,推动了分辨率的界限.
  • 该技术对于从生物巨分子中获得高分辨率的结构信息至关重要.
  • 进一步开发Cryo-LM有望更深入地了解细胞结构和功能.