中性粒细胞对SARS-CoV-2的杀毒活性是由中性粒细胞外细胞陷调解的
Cícero José Luíz Dos Ramos Almeida1,2, Flávio Protásio Veras1,2,3, Isadora Marques Paiva1,2
1Center for Research in Inflammatory Diseases.
The Journal of infectious diseases
|November 28, 2023
概括
中性粒细胞外细胞陷 (NETs) 捕获并抑制SARS-CoV-2在肺细胞和中性粒细胞中的复制. 降低NETs会增加病毒载量,突出显示NETosis是对抗COVID-19的关键防御.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- COVID-19肺炎涉及中性粒细胞和中性粒细胞外细胞陷 (NETs),可能导致组织损伤.
- 针对SARS-CoV-2的NET的杀毒活性在很大程度上仍未知.
研究的目的:
- 调查NET是否对SARS-CoV-2具有杀毒活性.
- 为了确定 NET 降解对 SARS-CoV-2 复制 in vivo 和 in vitro 的影响.
主要方法:
- 免疫光显微镜观察病毒颗粒和NETs.
- 在体外感染中性粒细胞和上皮细胞的SARS-CoV-2.
- 使用DNase I. 进行NET的退化.
- 感染K18人性化ACE2转基因小鼠的SARS-CoV-2.
主要成果:
- 病毒颗粒在受感染的中性粒细胞中与NETs共定位.
- 抑制NET的产生增加了SARS-CoV-2的复制.
- NETs抑制了病毒感染和在上皮细胞中的复制.
- DNase I 治疗降低了 NET 的杀病毒效应,并增加了小鼠的病毒载量.
- 网的病毒杀伤作用独立于中性粒细胞弹性酶或髓氧化酶.
结论:
- NETosis 在捕获和抑制SARS-CoV-2中发挥作用.
- NETs显示出对SARS-CoV-2的杀毒活性.
- NETs代表了对SARS-CoV-2感染的先天免疫反应的关键组成部分.
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