在体外成熟过程中补充阿尼索尔会增加体外山羊胚胎的产量,以度依赖的方式
A L Conceição-Santos1, A C A Ferreira1, N A R Sá1
1Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Theriogenology
|November 28, 2023
概括
在山羊卵细胞的体外成熟 (IVM) 过程中补充阿尼索尔显示出度依赖的效果. 低度 (30微克/毫升) 改善了卵细胞成熟,活力和胚胎生产,而高度 (2000微克/毫升) 是有害的.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 生物化学 生物化学
背景情况:
- 卵细胞的体外成熟 (IVM) 可以诱导氧化应激,影响成熟和活力.
- 醇是一种天然的烯胺,具有抗氧化特性,但其对山羊卵细胞IVM的影响尚未研究.
研究的目的:
- 评估不同度的乙醇对山羊卵细胞成熟,氧化应激和随后胚胎发育的影响.
- 为了确定在IVM期间改善羊卵细胞质量的最佳anethole度.
主要方法:
- 羊卵细胞复合体 (COCs) 在TCM-199+介质中成熟,并补充了0,30,300或2000μg/mL的anethole.
- 评估了卵细胞成熟,活力,染色质配置,活性氧物种 (ROS) 水平和线粒体膜潜力.
- 为了评估胚胎发育,进行了基激活和随后的7天体外培养.
主要成果:
- 与对照组相比,30μg/mL的阿尼索尔显著增加了卵细胞成熟,裂变率,活力和体外胚胎生产.
- 醇在2000μg/mL下降了成熟和裂解率和胚胎的生产.
- 高度的阿尼索尔 (2000微克/毫升) 增加了线粒体膜潜力,而30微克/毫升的度对整个卵细胞质量产生了有益影响.
结论:
- 乙醇对山羊卵细胞IVM和随后的胚胎发育产生度依赖的作用.
- 建议补充30μg/mL的乙醇,以提高卵细胞的活力,成熟和体外胚胎的生产.
- 高度的乙醇可能会对山羊的生殖结果产生负面影响.
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