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相关概念视频

CRISPR01:59

CRISPR

51.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
51.7K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K

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相关实验视频

Updated: Jul 9, 2025

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
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一个放射性CRISPR干扰系统使用Zr标记的LBCas12a.

Injoo Hwang1, Jun Young Lee1, Tae-Hyun Kim1

  • 1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Republic of Korea.

Journal of controlled release : official journal of the Controlled Release Society
|November 28, 2023
PubMed
概括

研究人员开发了同位素标记的CRISPR蛋白质,用于向药物输送和体内成像. 这种治疗方法在肝纤维化模型中显示出治疗效果,推进了CRISPR应用.

关键词:
这就是CRISPR干扰的原因.在LbCas12a中使用.放射性标记的CRISPR是一种放射性标记.放射性标记疗法是一种放射性标记疗法.-89 -89 -89 -89 -89 -89 -89 -89 -89 -89 -89

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科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 放射化学 放射化学是指辐射化学.

背景情况:

  • 克里斯普尔蛋白对药物开发有希望,但缺乏合适的输送系统.
  • 有针对性的药物输送需要敏感和稳定的药物在体内应用.
  • -89 (Zr) 为安全的实时体内追踪提供正电子发射.

研究的目的:

  • 评估Zr标签对CRISPR蛋白功能的影响.
  • 为了评估Zr标记的CRISPR蛋白的治疗潜力.
  • 在肝纤维化模型中使用CRISPR干扰来证明治疗疗效.

主要方法:

  • 用正子发射同位素标记CRISPR蛋白质89Zr.
  • 在体内行为成像以确认标记后的蛋白质功能.
  • 在肝纤维化的临床前小鼠模型中进行治疗评估.

主要成果:

  • 在体内成像过程中,Zr标签不会影响CRISPR蛋白的功能.
  • 在肝纤维化中证明了CRISPR干扰系统的治疗疗效.
  • 突出了同位素标记的CRISPR蛋白质的治疗能力.

结论:

  • 89 Zr标记的CRISPR蛋白质是功能性的,适用于体内追踪和治疗.
  • 这种治疗术的方法有可能用于治疗肝纤维化.
  • 这些发现支持对同位素标记的CRISPR蛋白质进行临床探索,用于体内应用.