在Hemidactylus flaviviridis中的卵巢内沙丁-1:生殖阶段依赖的表达,作用和荷尔蒙调节
Krittika Dotania1, Mamta Tripathy1, Umesh Rai2
1Department of Zoology, University of Delhi, Delhi 110007, India.
概括
涅斯法廷-1在壁卵巢中起着关键作用,影响细胞存活,增殖和类固醇生产. 这项研究揭示了其生殖阶段依赖的表达和荷尔蒙调节.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
背景情况:
- 众所周知,纳斯法廷-1 (nucleobindin 2/nesfatin-1) 能够调节哺乳动物的卵巢功能.
- 对非哺乳动物脊椎动物的有限研究表明,它在类固醇生成和卵细胞成熟中起作用,但它在爬行动物的功能尚未被探索.
- 墙壁 (Hemidactylus flaviviridis) 作为研究新型生殖的模型生物.
研究的目的:
- 在Hemidactylus flaviviridis的卵巢中识别nesfatin-1.
- 阐明纳斯法-1在卵巢功能中的作用,包括亡,增殖,类固醇生成和新陈代谢.
- 通过淋巴激素和性类固醇研究卵巢内沙丁-1的调节.
主要方法:
- 对不同生殖阶段和卵泡发育中的nukb2/nesfatin-1表达的定量分析.
- 在实验室内用nesfatin-1化壁卵巢,以评估对基因表达的影响 (bcl-2,caspase-3,star,scf,c-kit,pcna,bmp-15).
- 在纳斯法-1治疗后测量雌激醇和孕的产生,葡萄糖的摄取和新陈代谢.
- 激素调节研究涉及二测试 (DHT),17β-雌激醇 (E2) 和卵泡刺激激素 (FSH).
主要成果:
- 卵巢 nucb2 / nesfatin-1 表达在晚期复发和繁殖期间达到峰值,在previtellogenic毛囊中具有高水平.
- 纳斯法丁-1治疗增加了抗亡基因 (bcl-2) 表达,减少了亲亡基因 (caspase-3) 表达,并刺激了增殖标志物.
- 纳斯法-1增强了卵巢类固醇生成 (增加了雌激素,孕和明星表达),并刺激了葡萄糖的吸收和新陈代谢.
- 卵巢内沙丁-1表达被DHT和E2刺激,但被FSH抑制.
结论:
- 这项研究首次证实了纳斯法-1在爬行动物的卵巢功能.
- 纳斯法-1在调节壁卵巢中细胞亡,增殖,类固醇生成和新陈代谢方面发挥着重要作用.
- 卵巢内沙丁-1的表达是通过H. flaviviridis中的性类固醇和性腺激素进行差异调节的.
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