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相关概念视频

Colloidal precipitates01:09

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...

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A Modified Precipitation Method to Isolate Urinary Exosomes
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通过离子强度调制进行外体沉:外体

Vijaya Sunkara1, Juhee Park1, Jiyun Han1,2

  • 1Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.

ACS applied materials & interfaces
|November 28, 2023
PubMed
概括

我们开发了ExoPRISM,这是一种简单,低成本的方法,可以有效地从生物流体中分离细胞外囊泡 (EV). 这种技术提供了高产量和纯度,推进了液体活检和再生医学.

关键词:
电动汽车隔离 电动汽车隔离在EV降水量下降.电动汽车分离器 EV分离器电解质是一种电解质.外基因组是外基因组的组成部分.细胞外囊泡 (EV) 是一种

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科学领域:

  • 生物化学 生物化学
  • 生物技术是生物技术.
  • 纳米技术纳米技术

背景情况:

  • 细胞外囊泡 (EVs) 对theranostics至关重要,但难以分离.
  • 目前的电动汽车分离方法是低效的,昂贵的,并且无法扩展.
  • 改善EV隔离对于推进诊断和治疗至关重要.

研究的目的:

  • 介绍ExoPRISM,一种新的,具有成本效益和用户友好的电动汽车分离方法.
  • 为了证明使用ExoPRISM隔离的EV的高产量和纯度.
  • 为了验证ExoPRISM在各种生物流体类型中的适应性.

主要方法:

  • 通过离子强度调制 (ExoPRISM) 开发了EV沉.
  • 使用增量电解质添加的蛋白质和EVs的顺序沉.
  • 低速离心和洗用于分数EV分离.
  • 过程在不到一个小时内完成.

主要成果:

  • ExoPRISM实现了电动汽车的高产量和纯度.
  • 孤立的EV保留了它们的生物功能.
  • 该方法对各种生物液体 (血,血清,尿液,培养基) 有效.
  • ExoPRISM是可扩展和具有成本效益的.

结论:

  • ExoPRISM为现有的电动汽车分离技术提供了一个更优质的替代方案.
  • 这种方法支持下一代液体活检的开发.
  • ExoPRISM显示出再生医学应用的巨大潜力.