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在生殖器官发育过程中对GhpPLA基因家族进行全面的鉴定和功能性表征

Mingyang Wang¹, Dingyan Tian¹, Tengyu Li²

¹National Engineering Research Center of Cotton Biology Breeding and Industrial Technology, Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang, Henan, 455000, China.

BMC plant biology

|November 28, 2023

概括

与帕塔丁相关的聚酶A (pPLAs) 对于棉花的繁殖发育至关重要. 特定的基因,GhpPLA23和GhpPLA44,影响花粉活力,可能是诱导植物中单 haploid 后代的关键.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
生物化学生物化学

背景情况:

脂酶A (PLA) 是参与脂代谢的酶,在植物生长和发育中起着至关重要的作用.
与帕塔丁相关的脂酶A (pPLA) 家族在各种植物物种中具有多样化的功能.

研究的目的:

在Gossypium hirsutum (棉花) 中识别和描述PPLA基因家族.
调查特定的PPLA基因在棉花生殖发育中的作用及其对平分类诱导的潜力.

主要方法:

全基因组分析以识别包括棉花在内的13种植物物种中的PPLA基因.
基因表达分析,包括花粉表达模式.
使用病毒诱导基因沉默 (VIGS) 的功能验证和评估花粉活力和活性氧物种 (ROS) 水平.

主要成果:

鉴定了294种PPLA,其中46种在棉花 (GhpPLA) 中被分为三个子家族.
GhpPLAs主要表达在生殖器官,如花,花和花.
沉默GhpPLA23和GhpPLA44导致花粉活性降低和ROS水平改变,表明它们在生殖发育中的重要性.

结论:

pPLA基因,特别是GhpPLA23和GhpPLA44,对于棉花中的花器官和精子细胞发育至关重要.
这些基因在棉花生殖发育中起着重要作用.
在棉花中,ppLA基因代表诱导单 haploid 后代的潜在目标.

关键词:

棉花棉花棉花棉花棉花进化分析是一种进化分析.功能性表征的功能性表征生殖器官是一种生殖器官.VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS 在pPLAs中使用.

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